腺样囊性癌细胞与大鼠施万细胞共培养的实验研究

中国口腔颌面外科杂志 ›› 2015, Vol. 13 ›› Issue (6): 481-485.

• • 下一篇

腺样囊性癌细胞与大鼠施万细胞共培养的实验研究

单春，杨新杰，吴宝磊，王新革，雷德林

第四军医大学口腔医院军事口腔医学国家重点实验室，陕西西安 710032

收稿日期:2015-01-26 出版日期:2015-11-20 发布日期:2015-12-03
通讯作者: 雷德林，E-mail：leidelin@fmmu.edu.cn
作者简介:单春（1982- ），男，硕士研究生，E-mail：39538985@qq.com
基金资助:
Supported by National Natural Science Foundation of China (81372901, 81302352).

Research on the co-culture model of salivary adenoid cystic carcinoma cells and rat schwann cells

SHAN Chun, YANG Xin-jie, WU Bao-lei, WANG Xin-ge, LEI De-lin.

State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University. Xi'an 710032, Shaanxi Province, China

Received:2015-01-26 Online:2015-11-20 Published:2015-12-03
Contact: 国家自然科学基金（81372901，81302352）

摘要/Abstract

摘要： 目的：建立腺样囊性癌细胞与大鼠施万细胞共培养模型，探讨细胞间相互作用时BDNF、TrkB和E-cadherin的表达变化情况。方法：采用Transwell系统建立腺样囊性癌细胞与大鼠施万细胞共培养模型，共培养96 h后，观察肿瘤细胞的形态学变化，以ELISA法检测各组培养液中BDNF含量，实时定量PCR（RT-PCR）、Western免疫印迹检测肿瘤细胞中BDNF、TrkB及E-cadherin的表达情况。采用SPSS17.0软件包对实验结果进行统计学处理。结果：与施万细胞共培养的腺样囊性癌细胞发生长梭形及多角形改变，E-cadherin表达下调，TrkB表达上调，BDNF表达未见明显变化。与腺样囊性癌细胞系共培养的培养液中，施万细胞表达BDNF的量比单独培养的施万细胞显著增高；而对照组黏液表皮样癌细胞与施万细胞共培养时未发生类似改变。结论：BDNF/TrkB信号通路在唾液腺腺样囊性癌嗜神经侵袭过程中发挥重要作用，但其具体分子机制有待进一步研究。

关键词: 腺样囊性癌, 施万细胞, 共培养, BDNF, TrkB, E-cadherin, 嗜神经侵袭

Abstract: PURPOSE : To establish a co-culture system of salivary adenoid cystic carcinoma (SACC) cells with rat schwann cells for further investigating the changes of brain-derived neurotrophic factor (BDNF), tropomysin-related kinase B (TrkB) and E-cadherin after cells interaction. METHODS : Transwell system was used to establish co-culture model of SACC cells with rat schwann cells. After co-culture for 96 hours, the morphology of tumor cells were investigated, the change of BDNF in co-culture medium of each group was tested by enzyme-linked immunosorbent assay (ELISA) and the expression of BDNF, TrkB and E-cadherin in tumor cells were detected by RT-PCR and Western blot. The date was analyzed using SPSS 17.0 software package. RESULTS : Compared with the single culture group, the morphology of SACC cells co-cultured with schwann cells changed to spindle-shape and polygon-shape; the expression of E-cadherin in co-cultured SACC cells was down-regulated while the expression of TrkB and phospho-TrkB was up-regulated, but the expression of BDNF had no obvious change; more expression of BDNF was detected in the co-culture medium secreted by schwann cells. The mucoepidermoid carcinoma (MEC) cells in the control group, co-cultured with schwann cells, had no similar changes. CONCLUSIONS : BDNF/TrkB signal pathway plays an important role in the process of perineural invasion of SACC, but the concrete molecular mechanism needs further experimental investigation.

Key words: Salivary adenoid cystic carcinoma, Schwann cells, Co-culture, BDNF, TrkB, E-cadherin, Perineural invasion

中图分类号:

R739.8

单春，杨新杰，吴宝磊，王新革，雷德林. 腺样囊性癌细胞与大鼠施万细胞共培养的实验研究[J]. 中国口腔颌面外科杂志, 2015, 13(6): 481-485.

SHAN Chun, YANG Xin-jie, WU Bao-lei, WANG Xin-ge, LEI De-lin.. Research on the co-culture model of salivary adenoid cystic carcinoma cells and rat schwann cells[J]. China J Oral Maxillofac Surg, 2015, 13(6): 481-485.

参考文献

[1] Li L, Li Y, Wen Y, et al. Clinical analysis of salivary gland tumor cases in West China in past 50 years [J]. Oral Oncol, 2008,44(2):187-192.
[2] Barrett AW, Speight PM. Perineural invasion in adenoid cystic carcinoma of the salivary glands: a valid prognostic indicator? [J]. Oral Oncol, 2009, 45(11): 936-940.
[3] Ayala GE, Wheeler TM, Shine HD, et al. In vitro dorsal root ganglia and human prostate cell line interaction: redefining perineural invasion in prostate cancer[J]. Prostate, 2001, 49(3): 213-223.
[4] Torigoe K, Hashimoto K, Lundborg G. A role of migratory Schwann cells in a conditioning effect of peripheral nerve regeneration [J]. Exp Neurol, 1999, 160(1): 99-108.
[5] Dud?s J, Bitsche M, Schartinger V, et al. Fibroblasts produce brain-derived neurotrophic factor and induce mesenchymal transition of oral tumor cells[J]. Oral Oncol, 2011, 47(2): 98-103.
[6] 王维玺, 杨新杰, 贾森, 等. BDNF、TrkB和E-cadherin在唾液腺腺样囊性癌中的表达及意义[J]. 中国口腔颌面外科杂志, 2014, 12(4): 289-294.
[7] Tao Y. Isolation and culture of Schwann cells[J]. Methods Mol Biol, 2013, 1018: 93-104.
[8] 郭峰,吕春堂,周中华,等.腺样囊性癌神经侵袭动物模型建立的可行性和特异性研究[J]. 第二军医大学学报,2005,26(6):593-596.
[9] Lamouille S, Xu J, Derynck R. Molecular mechanisms of epithelial-mesenchymal transition [J]. Nat Rev Mol Cell Bio, 2014, 15(3): 178-196.
[10] Smith A, Teknos TN, Pan Q. Epithelial to mesenchymal transition in head and neck squamous cell carcinoma [J]. Oral Oncol, 2013, 49(4): 287-292.
[11] Chen C, Zimmermann M, Tinhofer I, et al. Epithelial-to-mesenchymal transition and cancer stem(-like) cells in head and neck squamous cell carcinoma[J]. Cancer Lett,2013,338(1):47-56.
[12] Binesh F, Akhavan A, Masumi O, et al. Clinicopathological review and survival characteristics of adenoid cystic carcinoma [J]. Indian J Otolaryngol Head Neck Surg, 2015, 67(Suppl 1): 62-66.
[13] Schneider MR, Kolligs FT. E-cadherin's role in development, tissue homeostasis and disease: insights from mouse models: tissue-specific inactivation of the adhesion protein E-cadherin in mice reveals its functions in health and disease [J]. Bioessays, 2015, 37(3): 294-304.
[14] Kupferman ME, Jiffar T, El-Naggar A, et al. TrkB induces EMT and has a key role in invasion of head and neck squamous cell carcinoma [J]. Oncogene, 2010, 29(14): 2047-2059.
[15] Jia S, Wang W, Hu Z, et al. BDNF mediated TrkB activation contributes to the EMT progression and the poor prognosis in human salivary adenoid cystic carcinoma[J]. Oral Oncol,2015,51(1): 64-70.
[16] Sasahira T, Ueda N, Yamamoto K, et al. Trks are novel oncogenes involved in the induction of neovascularization, tumor progression, and nodal metastasis in oral squamous cell carcinoma [J]. Clin Exp Metastas, 2013, 30(2): 165-176.

腺样囊性癌细胞与大鼠施万细胞共培养的实验研究

Research on the co-culture model of salivary adenoid cystic carcinoma cells and rat schwann cells

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	胡永, 王琼, 赵大勇, 叶金海. 下颌下腺腺样囊性癌高级别转化1例报告[J]. 中国口腔颌面外科杂志, 2023, 21(2): 206-208.
[2]	游元和, 田卓炜, 杜仲, 肖孟, 许贵松, 郑家伟, 王延安. 靶向YAP协同强化selumetinib抑制神经纤维瘤相关施万细胞生长的体外研究[J]. 中国口腔颌面外科杂志, 2022, 20(4): 347-353.
[3]	刘嘉靓, 杜仲, 郑家伟, 游元和, 王延安. 不同MEK1/2抑制剂对NF1敲低的施万细胞增殖活性的影响[J]. 中国口腔颌面外科杂志, 2020, 18(4): 302-307.
[4]	徐博雅, 杨子桧, 王珺, 雷德林, 杨新杰. CCL2和CCR2在唾液腺腺样囊性癌中的表达及意义[J]. 中国口腔颌面外科杂志, 2019, 17(4): 311-315.
[5]	任华健, 李家苏, 高红梅, 尹林. NDRG2对唾液腺腺样囊性癌增殖、侵袭的影响[J]. 中国口腔颌面外科杂志, 2019, 17(2): 112-116.
[6]	王珊珊, XiaohuiRausch-Fan, OlehAndrukov, 林毅, 林李嵩, 施斌. Emdogain对共培养成骨样细胞及内皮细胞成骨及成血管基因表达的影响[J]. 中国口腔颌面外科杂志, 2019, 17(1): 32-39.
[7]	李欢, 杨新杰, 王维戚, 雷德林. 应用RNA-Seq技术筛选唾液腺腺样囊性癌嗜神经侵袭相关差异表达基因[J]. 中国口腔颌面外科杂志, 2018, 16(2): 114-119.
[8]	夏亮, 胡宇华, 王丽珍, 顾挺, 李江, 田臻. 唾液腺恶性多形性腺瘤中CDH1基因启动子甲基化与E-cadherin表达的相关性分析[J]. 中国口腔颌面外科杂志, 2018, 16(2): 120-125.
[9]	王超, 黎婷, 严斐, 蔡文燕, 蒋星宇, 孙晋虎. 辛伐他汀联合干扰miR-21对唾液腺腺样囊性癌细胞迁移和侵袭的影响[J]. 中国口腔颌面外科杂志, 2018, 16(2): 132-137.
[10]	严斐，王超，黎婷，蔡文燕，孙晋虎，. miR-21对唾液腺腺样囊性癌细胞增殖和凋亡的影响及机制研究[J]. 中国口腔颌面外科杂志, 2017, 15(3): 209-213.
[11]	米静, 张明宾, 万光勇. 槲皮素对人腺样囊性癌ACC-M细胞增殖和凋亡的影响及机制研究[J]. 中国口腔颌面外科杂志, 2017, 15(2): 121-126.
[12]	盛夏涵, 曲禄瑶, 蔡协艺. 大鼠骨髓间充质干细胞与髁突软骨细胞共培养诱导软骨化分化的实验研究[J]. 中国口腔颌面外科杂志, 2016, 14(6): 490-494.
[13]	曾威, 卢文辉, 张志利, 赵小朋. miR-582-5p在唾液腺腺样囊性癌侵袭、转移中的作用[J]. 中国口腔颌面外科杂志, 2016, 14(5): 413-418.
[14]	夏亮, 张春叶, 胡宇华, 钱佳骏, 李江, 田臻. 启动子甲基化状况对唾液腺恶性多形性腺瘤体外细胞系细胞E-cadherin表达的影响[J]. 中国口腔颌面外科杂志, 2016, 14(4): 308-314.
[15]	蔡文燕, 严斐, 董晶, 孙晋虎. 辛伐他汀对唾液腺腺样囊性癌细胞生长及survivin表达的影响[J]. 中国口腔颌面外科杂志, 2016, 14(3): 213-217.