中国口腔颌面外科杂志 ›› 2017, Vol. 15 ›› Issue (2): 121-126.doi: 10.19438/j.cjoms.2017.02.005

• 论文 • 上一篇    下一篇

槲皮素对人腺样囊性癌ACC-M细胞增殖和凋亡的影响及机制研究

米静1, 张明宾2, 万光勇2*, *   

  1. 1.潍坊医学院 口腔医学院,山东 潍坊 261000;
    2.泰安市中心医院 口腔科,山东 泰安 271000
  • 收稿日期:2016-07-18 出版日期:2017-03-20 发布日期:2017-04-13
  • 通讯作者: 万光勇,E-mail: wgykq@163.com
  • 作者简介:米静(1990-),女,在读硕士研究生,E-mail:mijingkouqiang@126.com

Effects and mechanism of quercetin on proliferation and apoptosis of human adenoid cystic carcinoma cell line ACC-M

MI Jing1, ZHANG Ming-bin2, WAN Guang-yong2   

  1. 1.Department of Stomatology, Weifang Medical University. Weifang 261000;
    2.Department of Stomatology, Tai'an City Central Hospital. Tai'an 271000, Shandong Province, China
  • Received:2016-07-18 Online:2017-03-20 Published:2017-04-13
  • Contact: 山东省泰安市科技发展计划(2015NS2099)

摘要: 目的 观察槲皮素对人腺样囊性癌细胞系ACC-M增殖和凋亡的作用,探讨槲皮素影响ACC-M细胞凋亡的可能机制。方法 采用CCK-8 法检测不同浓度的槲皮素对ACC-M细胞增殖的抑制作用并计算抑制率,计算IC50;流式细胞术检测 ACC-M细胞凋亡率和细胞周期分布;实时荧光定量RT-PCR检测 ACC-M细胞中Survivin mRNA的表达。采用SPSS 20.0软件包对数据进行统计学分析。结果 槲皮素抑制 ACC-M细胞增殖的作用明显,且呈浓度依赖性,IC50为151.12 μmol/L(P<0.05);Annexin V/PI 双染法检测显示,槲皮素能够诱导ACC-M细胞凋亡,且与剂量、时间呈正相关(P<0.05);PI染色法检测显示,槲皮素主要作用于 G2/M 节点,能将细胞特异性地阻滞于 G2/M 期;RT-PCR检测显示,随着药物浓度增加,ACC-M细胞中Survivin mRNA的表达水平均呈不同程度的降低,200 μmol/L 槲皮素可显著抑制Survivin mRNA的表达。结论 槲皮素能够抑制ACC-M细胞增殖,诱导其凋亡,且呈时间-剂量依赖性。槲皮素可能通过下调Survivin的表达水平而发挥抗腺样囊性癌的作用。

关键词: 槲皮素, 腺样囊性癌, 细胞增殖, 细胞凋亡, Survivin

Abstract: PURPOSE : To observe the effect of quercetin on human adenoid cystic carcinoma cells (ACC-M) proliferation and apoptosis. METHODS : CCK-8 staining was used to detect different concentrations of quercetin on ACC-M cell proliferation inhibition, the inhibition rate and IC50 were calculated; flow cytometry was used to detect ACC-M cell apoptosis rate and cell cycle distribution; Survivin mRNA of ACC-M cells was detected by RT-PCR. The difference between each group was analyzed with SPSS20.0 software package. RESULTS : Quercetin inhibited ACC-M cell growth obviously in a concentration dependent manner. The IC50 was 151.12 μmol/L (P<0.05); annexin V/PI double staining assay showed that quercetin can induce apoptosis of ACC-M cells in a dose and time dependent manner (P<0.05); PI staining assay showed that quercetin played an important role in G2/M cells, which were specifically arrested in G2/M phase. RT-PCR showed the quercetin concentration was negatively related with survivin mRNA in ACC-M cells and 200 μmol/L quercetin could inhibit the expression of Survivin mRNA significantly. CONCLUSION S: Quercetin can inhibit the proliferation of ACC-M cells and induce its apoptosis in a time and dose dependent manner by down-regulating the level of Survivin.

Key words: Quercetin, Adenoid cystic carcinoma, Cell proliferation, Cell apoptosis, Survivin

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