巨噬细胞来源的白细胞介素6对口腔鳞癌细胞增殖活性的影响

doi:10.19438/j.cjoms.2019.06.004

中国口腔颌面外科杂志 ›› 2019, Vol. 17 ›› Issue (6): 503-507.doi: 10.19438/j.cjoms.2019.06.004

巨噬细胞来源的白细胞介素6对口腔鳞癌细胞增殖活性的影响

李捷, 陈巨峰, 冼淡

佛山市第一人民医院口腔科,广东佛山 528000

收稿日期:2019-04-17 出版日期:2019-11-20 发布日期:2019-12-16
通讯作者: 李捷,E-mail:betty-chow@163.com
作者简介:李捷（1974-）,男,大专,主治医师

Macrophage-derived IL-6 promotes oral cancer cell proliferation

LI Jie, CHEN Ju-feng, XIAN Dan

Department of Stomatology, The First People′s Hospital of Foshan. Foshan 528000, Guangdong Province, China

Received:2019-04-17 Online:2019-11-20 Published:2019-12-16

摘要/Abstract

摘要： 目的: 探讨巨噬细胞来源的白细胞介素6（interleukin 6,IL-6）对口腔鳞状细胞癌细胞系增殖活性的影响。方法: 通过MTT法检测不同浓度重组IL-6对口腔鳞状细胞癌细胞系SCC-15和CAL-27增殖活性的影响。建立佛波酯诱导的THP-1巨噬细胞分化模型,分别通过实时定量荧光PCR（RT-PCR）和酶联免疫吸附试验,分析脂多糖（lipopolysaccharide,LPS）刺激下的THP-1细胞中mRNA水平的变化以及THP-1细胞培养基中IL-6的含量。利用MTT法检测含THP-1条件培养基对SCC-15和CAL-27细胞系增殖活性的影响。采用GraphPad 7.0软件包对数据进行统计学分析。结果: 培养基中添加IL-6,可促进SCC-15和CAL-27细胞系的增殖活性。LPS可刺激活化的THP-1巨噬细胞分泌IL-6;活化的THP-1细胞培养基可促进SCC-15和CAL-27细胞的增殖活性。结论: LPS刺激巨噬细胞分泌的IL-6,可促进口腔鳞状细胞癌细胞系SCC-15和CAL-27的增殖活性。

关键词: 巨噬细胞, 白细胞介素6, 口腔癌, 增殖

Abstract: PURPOSE: To investigate the effect of macrophage-derived IL-6 on oral cancer cell proliferation. METHODS: The proliferation rates of SCC-15 and CAL-27 cells under different concentration of IL-6 were measured by MTT assay. THP-1 macrophages were induced by PMA, IL-6 mRNA level in LPS-stimulated THP-1 cells were measured by real-time qPCR, IL-6 protein contents in THP-1 conditional medium were measured by ELISA. SCC-15 and CAL-27 cells were co-cultured with THP-1 conditional medium, cell proliferation rate was measured by MTT assay. The data were analyzed by GraphPad 7.0 software package. RESULTS: Recombinant IL-6 in culture medium promoted SCC-15 and CAL-17 cell proliferation; LPS stimulated IL-6 expression in PMA-induced THP-1 macrophages. THP-1 conditional medium promoted SCC-15 and CAL-27 cell proliferation. CONCLUSIONS: IL-6 derived by LPS-stimulated macrophages could promote SCC-15 and CAL-27 cell proliferation.

Key words: Macrophage, Interleukin 6, Oral cancer, Proliferation

中图分类号:

R739.8

李捷, 陈巨峰, 冼淡. 巨噬细胞来源的白细胞介素6对口腔鳞癌细胞增殖活性的影响[J]. 中国口腔颌面外科杂志, 2019, 17(6): 503-507.

LI Jie, CHEN Ju-feng, XIAN Dan. Macrophage-derived IL-6 promotes oral cancer cell proliferation[J]. China J Oral Maxillofac Surg, 2019, 17(6): 503-507.

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巨噬细胞来源的白细胞介素6对口腔鳞癌细胞增殖活性的影响

Macrophage-derived IL-6 promotes oral cancer cell proliferation

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