关键词: 组织工程骨, 牙槽突裂, 支架材料, BMP-2, 冻干法

Abstract: PURPOSE: To load BMP-2 on a novel inorganic ceramic scaffold material, OsteoBoneTM, through lyophilization, and explore its effect on bone formation after bone graft in vivo. METHODS: BMP-2 was loaded onto OsteoBoneTM scaffolds by coating it via lyophilization with trehalose added as stabalizer. The samples were incubated in PBS in vitro and the release rate was examined with BCA method and alkaline phosphatase (ALP) activity was detected. Eighteen SD rats were used as experimental animals. Critical size defects (CSD) of 4 mm×3 mm×3 mm were established on maxillary alveolar bone in SD rats. Scaffolds alone (group A), BMSC-scaffolds (group B)and BMP-2-scaffolds (group C) were used to be grafted into CSDs. Micro-CT and H-E staining were performed respectively 3,6 weeks post-surgery. SPSS 19.0 software package was used for statistical analysis. RESULTS: BMP-2 performed sustained release and maintained bioactivity for enhanced osteogenesis from lyophilized OsteoBoneTM scaffolds in vitro. Three weeks after surgery, H-E staining showed sparse braided bone, and there was no significant difference between the three groups; there was no significant difference in micro-CT analysis of bone volume/tissue volume (BV/TV) and trabecular thickness. Six weeks after surgery, H-E staining revealed abundant bone regeneration and osseous tissue was mature in group B and group C, and new bone formation trend was more apparent than in group A. Micro-CT analysis showed that BV/TV and trabecular thickness in group B and C was significantly greater than that in group A, and there was no significant difference between group B and group C. CONCLUSIONS: Loading BMP-2 on OsteoBoneTM scaffolds through lyophilization can significantly enhance the osteogenic capacity and achieve a similar effect to mesenchymal stem cells.

Key words: Tissue engineering bone, Alveolar cleft, Scaffold, BMP-2, Lyophilization