Effects of puerarin on osteoclast differentiation of marrow-derived macrophages from mandible

doi:10.19438/j.cjoms.2019.03.001

China Journal of Oral and Maxillofacial Surgery ›› 2019, Vol. 17 ›› Issue (3): 193-197.doi: 10.19438/j.cjoms.2019.03.001

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Effects of puerarin on osteoclast differentiation of marrow-derived macrophages from mandible

MA Xu-hui¹, ZHANG Peng², YANG Yi-ling³, XU Hong-yuan³, GONG Xin-yi³, ZHOU Si-ru³, JIANG Ling-yong³, DAI Qing-gang²

1.Department of Oromaxillofacial Head and Neck Oncology;
2.The 2nd Dental Centre;
3.Department of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine;National Clinical Research Center for Oral Diseases;Shanghai Key Laboratory of Stomatology &Shanghai Research Institute of Stomatology. Shanghai 200011, China;

Received:2019-01-16 Revised:2019-03-15 Online:2019-05-20 Published:2019-06-21

Abstract

Abstract: PURPOSE: To study the effect of puerarin on osteoclast differentiation of marrow-derived macrophages (BMMs) from mandible. METHODS: BMMs were obtained from 4-week-old female SD rats. Cell viability of BMMs with different concentration of puerarin was determined by CCK-8. Tartrate resistant acid phosphatase staining and q-PCR were used to analyse the changes of osteoclast formation and expression of osteoclast gene of BMMs due to different concentration of puerarin. Statistical analysis was performed using SPSS 16.0 software package. RESULTS: Cell viability of BMMs with puerarin at 1 nmol/L, 10 nmol/L, 100 nmol/L, 1 μmol/L and 10 μmol/L showed no difference from the control group, and 100 μmol/L group displayed decreased cell viability (P<0.05). Osteoclast number and relative osteoclast surface reduced in BMMs with 10 nmol/L, 100 nmol/L and 1 μmol/L puerarin in comparison with the control group. Furthermore, the expression of NFATc1, C-fos, TRAP and CTSK deceased in BMMs with 10 nmol/L, 100 nmol/L, and 1 μmol/L puerarin. CONCLUSIONS: Puerarin could impede osteoclast differentiation of mandibular BMMs, which may be used to prevent and treat osteoporosis in alveolar and jaw bone.

Key words: Puerarin, Osteoporosis, Mandible bone marrow-derived macrophages, Osteoclast

CLC Number:

R739.8

MA Xu-hui, ZHANG Peng, YANG Yi-ling, XU Hong-yuan, GONG Xin-yi, ZHOU Si-ru, JIANG Ling-yong, DAI Qing-gang. Effects of puerarin on osteoclast differentiation of marrow-derived macrophages from mandible[J]. China Journal of Oral and Maxillofacial Surgery, 2019, 17(3): 193-197.

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Effects of puerarin on osteoclast differentiation of marrow-derived macrophages from mandible

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