miRNA-32-5p target regulating EZH2 and its effect on proliferation of human oral carcinoma cell line CAL-27

Abstract

Abstract: PURPOSE: To construct a lentiviral vector of miR- 32-5p and verify its targeted effect on EZH2 gene and its effect on the proliferation of CAL-27 cells. METHODS: pSicoR-miR-32-5p and pMIR-Report-EZH2 over-expression vector were constructed. The targeted effect of miR-32-5p on EZH2 gene was verified by relative luciferase activity, Western blot and real-time PCR test. The effects on CAL-27 cells proliferation of over-expression miR-32-5p were analyzed by MTT assay. SPSS 20.0 software package was used for statistical analysis. RESULTS: The recombinant plasmids of pSicoR-miR- 32-5p and pMIR -Report-EZH2 3'-UTR were constructed successfully. Over-expression of miR-32-5p suppressed the mRNA and protein expression level of EZH2 significantly (P<0.05). Suppression of miR-32-5p expression increased the expression level of EZH2 mRNA and protein significantly(P<0.05).MTT assay showed that the proliferation effect of lentivirus vector of miR-32-5p on CAL-27 cells were significant(P<0.05). CONCLUSIONS: MiR-32-5p can suppress EZH2 gene expression by targeting the specific sequence of EZH2-3'UTR and affect proliferation of human oral carcinoma cell line CAL-27.

Key words: miRNA-32-5p, Oral carcinoma, EZH2, CAL-27

CLC Number:

R392
R78

GUO Ming, HAO Wen-hui, ZHAI Xin-ying, HOU Lin-yan. miRNA-32-5p target regulating EZH2 and its effect on proliferation of human oral carcinoma cell line CAL-27[J]. China Journal of Oral and Maxillofacial Surgery, 2016, 14(2): 111-116.

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