Establishment and histological evaluation of traumatic temporomandibular joint model in goat

Abstract

Abstract: PURPOSE: To establish traumatic temporomandibular joint model in goat, and evaluate the histopathological changes. METHODS: According to the clinical traumatic type, 16 adult goats’ bilateral joints were randomly divided into 4 groups following surgical procedures. Disc displacement without reduction (DD): the attachment of disc was severed, and pulled anteriorly; the disc was fixed in the root of coronoid process. Condylar cartilage defect (CCD): the inferior compartment of TMJ was open to grind off lateral 1/3 condylar cartilage. Disc displacement and condylar cartilage defect (DD+CCD): the above two METHODS were used. Blank control group(C): nothing was done in TMJ. The TMJs were removed and observed 2 weeks and 1 month after surgery. The histopathological score of condylar cartilage was observed and was statistically analysed between each two groups (4 groups) with SPSS 17.0 software package. RESULTS: DD+CCD showed fibrous adhesion and coarse condylar cartilage; histologically, the composition of condylar cartilage was in disorder; and matrix proteoglycan decreased at 2-week and 1-month. Partial cartilage desquamation and fibrous adhesion exacerbated at 1-month. DD showed disordered cartilage composition, intact superfacial zone, little angiogenesis and proteoglycan degradation at 2-week and 1-month. CCD showed fibrosis between disc and defective cartilage in defective condyle, and the remnant cartilage composition and proteoglycan were normal at 2-week and 1-month. Cartilage degeneration was more serious at 1-month than 2-week in each group; and cartilage score was significantly different (P<0.05)in the following order: DD+CCD(11.75±0.75),DD(8.75±0.25),CCD(1.75±0.85),and C(0.00±0.00). CONCLUSION: Three traumatic TMJ models in goat were successfully established. The degeneration of cartilage in DD+CCD was most severe, and CCD was the mildest.

Key words: Temporomandibular joint, Disc displacement, Condylar cartilage defect, Traumatic osteoarthritis, Animal model

LI Ling-zhi, WANG Li-zhen, HE Dong-mei, YANG Chi. Establishment and histological evaluation of traumatic temporomandibular joint model in goat[J]. China Journal of Oral and Maxillofacial Surgery, 2014, 12(1): 13-19.

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