化学致癌剂4-NQO饮水法诱导小鼠口腔鳞癌模型的建立

doi:10.19438/j.cjoms.2019.04.003

中国口腔颌面外科杂志 ›› 2019, Vol. 17 ›› Issue (4): 300-303.doi: 10.19438/j.cjoms.2019.04.003

化学致癌剂4-NQO饮水法诱导小鼠口腔鳞癌模型的建立

傅友¹, 许可¹, 李江², 孙树洋¹, 张志愿¹

1.上海交通大学医学院附属第九人民医院·口腔医学院口腔颌面-头颈肿瘤科;
2.口腔病理科,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011

收稿日期:2019-01-10 修回日期:2019-03-28 出版日期:2019-07-20 发布日期:2019-08-12
通讯作者: 张志愿,E-mail：zhzhy@sjtu.edu.cn
作者简介:傅友（1992-）,男,在读硕士研究生,E-mail496932394@qq.com
基金资助:
国家重点研究开发项目(2017YFC0908500); 国家博士后科学基金（2017M610264）

Establishment of an oral squamous cell carcinoma model by using chemical carcinogen 4-NQO

FU You¹, XU Ke¹, LI Jiang², SUN Shu-yang¹, ZHANG Zhi-yuan¹

1. Department of Oromaxillofacial Head and Neck Oncology;
2.Department of Oral Pathology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institution of Stomatology. Shanghai 200011, China;

Received:2019-01-10 Revised:2019-03-28 Online:2019-07-20 Published:2019-08-12

摘要/Abstract

摘要： 目的：使用化学致癌剂4-硝基喹啉-1-氧化物（4-nitro-quinoline 1-oxide,4-NQO）诱导并建立C57BL/6小鼠口腔鳞癌病变模型。方法将4-NQO加入小鼠饮水,观察小鼠口腔黏膜的变化。于第12、16、20、24周处死,采集病变组织的样本,确定病变性质,并分离、培养肿瘤细胞。结果实验组小鼠在16周后口腔黏膜有明显的外生性肿物发生,直径约0.2 mm。小鼠口腔黏膜肿物H-E染色结果显示,肿物呈外生性或浸润性生长,可见角化珠和明显的核分裂象。细胞呈多边形,贴壁牢固,CK、Ki-67染色阳性,表明此细胞为鳞癌细胞。结论该模型诱导过程与人口腔鳞癌发病过程相似,是理想的研究口腔鳞癌发生、发展机制的动物模型。

关键词: 口腔鳞癌, 4-NQO, 动物模型, 细胞系

Abstract: PURPOSE: To establish a murine model with oral squamous cell carcinoma（OSCC）induced by chemical carcinogen 4-NQO. Methods: 4-NQO was added to drinking water for C57BL/6 mice, and the changes of oral mucosa were observed. The mice were sacrificed at various time points (12, 16, 20, 24 weeks), and the tissue samples were collected for identifying the characteristics and primary cell culture. Results: Mice in the experimental group had obvious exogenous tumors in the oral mucosa after 16 weeks, their diameters were about 0.2 mm. H-E staining of the tumors showed exogenous or invasive growth, with obvious keratinization and mitosis. The cells were polygonal and adherent to the wall firmly. CK and ki-67 staining was positive, indicating that the cells were squamous cell carcinoma cells. Conclusions: The induction process of this model is similar to that of human OSCC development, and it is an ideal animal model to study the mechanism of OSCC.

Key words: Oral squamous cell carcinoma, 4-NQO, Animal model, Cell line

中图分类号:

R739.8

傅友, 许可, 李江, 孙树洋, 张志愿. 化学致癌剂4-NQO饮水法诱导小鼠口腔鳞癌模型的建立[J]. 中国口腔颌面外科杂志, 2019, 17(4): 300-303.

FU You, XU Ke, LI Jiang, SUN Shu-yang, ZHANG Zhi-yuan. Establishment of an oral squamous cell carcinoma model by using chemical carcinogen 4-NQO[J]. China J Oral Maxillofac Surg, 2019, 17(4): 300-303.

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化学致癌剂4-NQO饮水法诱导小鼠口腔鳞癌模型的建立

Establishment of an oral squamous cell carcinoma model by using chemical carcinogen 4-NQO

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