唾液腺黏液表皮样癌中CLIC3的表达及意义

中国口腔颌面外科杂志 ›› 2016, Vol. 14 ›› Issue (3): 239-243.

唾液腺黏液表皮样癌中CLIC3的表达及意义

王智明¹, 温惠媛¹, 叶明², 杨东升¹, 张维庆¹, 潘遥¹, 马颖³

1.中国医科大学附属盛京医院口腔颌面外科,辽宁沈阳 110004;
2.抚顺矿务局总医院口腔科,辽宁抚顺 113008; 3.中国医科大学附属盛京医院病理科,辽宁沈阳 110004

收稿日期:2016-01-13 出版日期:2016-06-20 发布日期:2016-07-04
通讯作者: 王智明,E-mail: wangzm@sj-hospital.org
作者简介:王智明（1972-）,男,博士,副教授,硕士研究生导师
基金资助:
辽宁省科学技术计划项目（2012225100）

Expression and significance of CLIC3 in salivary gland mucoepidermoid carcinoma

WANG Zhi-ming¹, WEN Hui-yuan¹, YE Ming², YANG Dong-sheng¹, ZHANG Wei-qing¹, PAN Yao¹, MA Ying³

1.Department of Oral and Maxillofacial Surgery, Shengjing Hospital of China Medical University. Shenyang 110004; 2. Department of Stomatology, Fushun General Hospital of Coal Mine Bureau. Fushun 113008; 3. Department of Pathology, Shengjing Hospital of China Medical University. Shenyang 110004, Liaoning Province, China）

Received:2016-01-13 Online:2016-06-20 Published:2016-07-04

摘要/Abstract

摘要： 目的探讨细胞内氯离子通道蛋白3 (chloride intracellular channel protein 3, CLIC3)在唾液腺黏液表皮样癌(mucoepidermoid carcinoma, MEC)中的表达及临床意义。方法采用免疫组织化学和Western 免疫印迹方法检测MEC细胞株M3SP2和MC3中CLIC3的表达情况。应用免疫组织化学方法检测49例大唾液腺和18例小唾液腺MEC组织中CLIC3的表达,采用SPSS13.0 软件包对数据进行统计学分析。结果CLIC3在黏液表皮样癌组织中的阳性表达率为83.6%,正常唾液腺组织中无阳性表达（P<0.01）。CLIC3的高表达与唾液腺MEC的临床病理参数之间无显著相关性（P>0.05）。黏液表皮癌细胞株M3SP2和MC3均阳性表达 CLIC3蛋白。结论CLIC3可能在MEC的发生与发展中发挥一定作用。

关键词: 细胞内氯离子通道蛋白3, 唾液腺, 黏液表皮样癌

Abstract: PURPOSE: To investigate the expression and significance of chloride intracellular channel protein 3 (CLIC3) in salivary gland mucoepidermoid carcinoma (MEC). METHODS: Immunohistochemical and Western blot method were used to detect the expression of CLIC3 in MEC M3SP2 and MC3 cell lines. Immunohistochemical method was used to detect the expression of CLIC3 in 49 major salivary glands and 18 minor salivary glands MEC tissues. SPSS 13.0 software package was used for statistical analysis. RESULTS: The positive expression rate of CLIC3 in salivary gland MEC tissues was 83.6%, whereas none in normal salivary gland tissues (P<0.01). High expression of CLIC3 was not related to clinicopathological parameters of salivary gland MEC(P>0.05). The expression of CLIC3 protein was positive in MEC M3SP2 and MC3 cell lines. CONCLUSIONS: CLIC3 may play a role in carcinogenesis and progress of salivary gland MEC.

Key words: Chloride intracellular channel protein 3, Salivary gland, Mucoepidermoid carcinoma

中图分类号:

R739.87

王智明, 温惠媛, 叶明, 杨东升, 张维庆, 潘遥, 马颖. 唾液腺黏液表皮样癌中CLIC3的表达及意义[J]. 中国口腔颌面外科杂志, 2016, 14(3): 239-243.

WANG Zhi-ming, WEN Hui-yuan, YE Ming, YANG Dong-sheng, ZHANG Wei-qing, PAN Yao, MA Ying. Expression and significance of CLIC3 in salivary gland mucoepidermoid carcinoma[J]. China J Oral Maxillofac Surg, 2016, 14(3): 239-243.

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