中国口腔颌面外科杂志

中国口腔颌面外科杂志 ›› 2023, Vol. 21 ›› Issue (2): 112-118.doi: 10.19438/j.cjoms.2023.02.002

• 论著 • 上一篇    下一篇

微纳米磷酸钛涂层促进钛植入体骨结合的实验研究

孙祎昕, 祝颂松, 姜楠   

  1. 口腔疾病研究国家重点实验室,国家口腔疾病临床医学研究中心, 四川大学华西口腔医院 正颌及关节外科,四川 成都 610041
  • 收稿日期:2022-09-26 修回日期:2022-12-20 出版日期:2023-03-20 发布日期:2023-06-12
  • 通讯作者: 姜楠,E-mail：dent_jn@163.com
  • 作者简介:孙祎昕（1996-）,男,硕士,E-mail： syx_1213@foxmail.com
  • 基金资助:
    国家自然科学基金（81901026）

Micro-nano titanium phosphate coating promoted osseointegration of titanium material

SUN Yi-xin, ZHU Song-song, JIANG Nan   

  1. State Key Laboratory of Oral Diseases; National Clinical Research Center for Oral Diseases; Department of Orthognathic and Temporomandibular Joint Surgery, West China Hospital of Stomatology, Sichuan University. Chengdu 610041, Sichuan Province, China
  • Received:2022-09-26 Revised:2022-12-20 Online:2023-03-20 Published:2023-06-12

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摘要： 目的： 探讨在钛（Ti）植入体表面制备微纳米磷酸钛涂层以促进骨结合的效果。方法： 通过特定条件的磷酸水热反应,制备出微纳米磷酸钛涂层的钛植入体,即微/纳米棒状涂层钛（micro/nanorod Ti, P-R-Ti）,对照组为未处理的光滑纯钛（cp-Ti）。对材料进行成分、形貌、亲水性等表征分析,并在体外条件下测试细胞增殖活力,分析与细胞黏附、分化相关蛋白及基因表达的变化,探讨P-R-Ti对大鼠骨髓间充质干细胞（BMSCs）生物学行为的影响,评估P-R-Ti植入大鼠胫骨12周后对骨结合的影响,并对其生物力学强度进行测试。采用SPSS 21.0软件包对数据进行统计学分析。结果： 材料表征表明,P-R-Ti表面具有密集的微纳米级棒状结构。体外实验表明,P-R-Ti组黏附相关蛋白及mRNA表达上调,细胞活力显著增强,ALP活性、Runx-2、Col-1、OPN、OCN mRNA的表达水平显著高于cp-Ti组。体内实验表明,P-R-Ti组骨面积比、骨骼与植入物之间的接触显著高于cp-Ti组,其最大推出力可达（179.3±11.3）N,极限抗剪切强度可达（19.25±1.85）N/mm2。结论： P-R-Ti可有效促进钛植入体的骨结合能力,有望作为一种良好的钛表面改性方法应用于临床。

关键词: 微/纳米多级界面, 骨结合, 植入体, 磷酸钛

Abstract: PURPOSE: To fabricate micro-nano titanium phosphate coating on the surface of titanium (Ti) material to promote osseointegration. METHODS: Through the hydrothermal reaction of phosphoric acid under specific conditions, a Ti sheet/cylinder with a coating of micro-nano titanium phosphate, namely micro/nanorod Ti (P-R-Ti), was prepared. The control group was untreated smooth pure Ti (cp-Ti). The materials were characterized and analyzed in terms of composition, morphology, hydrophilicity, etc., and the cell proliferation activity was tested in vitro. The changes in protein and gene expression related to cell adhesion and differentiation were analyzed to explore the effect of P-R-Ti on the biological behavior of rat bone marrow mesenchymal stem cells (BMSCs). At the same time, the effects of P-R-Ti on bone union were evaluated 12 weeks after implantation into rat tibia, and its biomechanical strength was tested.SPSS 21.0 software package was used for data analysis. RESULTS: The material characterization showed that the surface of P-R-Ti had a dense micro nano rod structure. In vitro experiments showed that the expression of adhesion related proteins and mRNA in P-R-Ti group was up-regulated, and the cell viability was significantly enhanced. The expression levels of ALP activity, Runx-2, Col-1, OPN, OCN mRNA in P-R-Ti group were significantly higher than those in cp Ti group. In vivo experiments showed that the bone area ratio and the contact between bone and implant in P-R-Ti group were significantly higher than those in cp-Ti group. The maximum pushing force was(179.3±11.3)N, and the ultimate shear strength was (19.25±1.85)N/mm2. CONCLUSIONS: P-R-Ti can effectively promote the osseointegration of titanium material. It is expected to be used as an excellent method of titanium surface modification for clinical application.

Key words: Micro/nano interface, Osseointegration, Implant, Titanium phosphate

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