中国口腔颌面外科杂志

中国口腔颌面外科杂志 ›› 2021, Vol. 19 ›› Issue (3): 213-216.doi: 10.19438/j.cjoms.2021.03.005

• 论著 • 上一篇    下一篇

雷洛昔芬对骨质疏松性颌骨骨折大鼠骨折愈合及OPG/RANKL/RANK系统的影响

李剑平1, 刘培1, 高学舸1, 石燕萍2   

  1. 1.唐山市协和医院 口腔外科,河北 唐山 063000;
    2.唐山市工人医院 内分泌一科,河北 唐山 063000
  • 收稿日期:2020-12-02 修回日期:2020-12-31 发布日期:2021-07-16
  • 通讯作者: 李剑平,E-mail: 765521795@qq.com
  • 作者简介:李剑平(1977-),男,硕士,副主任医师

Effects of raloxifene on fracture healing and OPG/RANKL/RANK system in rats with osteoporotic jaw fracture

LI Jian-ping1, LIU Pei1, GAO Xue-ge1, SHI Yan-ping2   

  1. 1. Department of Oral Surgery, Tangshan Union Medical College Hospital. Tangshan 063000, Hebei Province;
    2. The First Department of Endocrinology, Tangshan Gongren Hospital. Tangshan 063000, Hebei Province, China
  • Received:2020-12-02 Revised:2020-12-31 Published:2021-07-16

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摘要： 目的 分析雷洛昔芬对骨质疏松性颌骨骨折大鼠骨折愈合及骨保护蛋白（OPG）/核因子κB的受体活化因子配体（RANKL）/核因子κB的受体活化因子（RANK）系统的影响。方法 采用SPF级SD大鼠建立骨质疏松性颌骨骨折模型。建模成功后,以雷洛昔芬12 mg/kg灌胃干预4周。X线摄片检查骨折愈合情况,双能X线骨密度测量仪测量骨痂区骨密度（BMD）,H-E染色检查骨痂区骨组织形态,Western 免疫印迹检测OPG、RANKL、RANK蛋白表达情况。采用SPSS 22.0软件包对数据进行统计学分析。结果 雷洛昔芬组术后2周、4周骨折线恢复较模型组优,H-E染色显示骨痂区骨组织骨小梁、骨基质样团块较模型组多,术后4周与空白对照组相当。雷洛昔芬组术后2、4周骨痂区BMD及OPG蛋白相对表达量显著高于模型组,RANKL、RANK蛋白相对表达量显著低于模型组（P<0.05）,术后4周与空白对照组相当（P>0.05）。结论 雷洛昔芬能调节OPG/RANKL/RANK系统,促进骨质疏松性颌骨骨折大鼠的骨折愈合。

关键词: 雷洛昔芬, 骨质疏松, 颌骨骨折

Abstract: PURPOSE: To investigate the effects of raloxifene on fracture healing and osteoprotegerin (OPG) / receptor activator of NF-κB ligand (RANKL) / receptor activator of NF-κB (RANK) system in rats with osteoporotic jaw fracture. METHODS: SPF SD rats were used to establish an osteoporotic jaw fracture model. After successful establishment of the model, 12 mg/kg raloxifene were administered intragastrically for 4 weeks. X-ray film was taken to check the fracture healing. Bone mineral density (BMD) was measured by dual energy X-ray absorptiometry. H-E staining was used to examine the morphology of callus. Western blot was used to detect the expression of OPG, RANKL and RANK. SPSS 22.0 software package was used for data analysis. RESULTS: The fracture line recovery of the raloxifene group was better than that of the model group 2 and 4 weeks after surgery. H-E staining showed more bone trabecula and bone matrix-like masses in the callus area than the model group, which was similar to that in blank control group 4 weeks after operation. The BMD and relative expression of OPG protein in raloxifene group were significantly higher than those in model group at 2 and 4 weeks after operation, and the relative expression of RANKL and RANK protein were significantly lower than those in model group (P<0.05), which were similar to those in blank control group 4 weeks after operation (P>0.05). CONCLUSIONS: Raloxifene can regulate OPG/RANKL/RANK system and promote fracture healing in rats with osteoporotic jaw fracture.

Key words: Raloxifene, Osteoporosis, Jaw fracture

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