间歇性鼻阻塞对幼年大鼠髁突软骨细胞成软骨作用的早期影响

doi:10.19438/j.cjoms.2018.02.008

中国口腔颌面外科杂志 ›› 2018, Vol. 16 ›› Issue (2): 138-143.doi: 10.19438/j.cjoms.2018.02.008

间歇性鼻阻塞对幼年大鼠髁突软骨细胞成软骨作用的早期影响

孙蕙珺, 王晓玲, 朱妍菲, 于倩, 聂萍, 朱敏

上海交通大学医学院附属第九人民医院·口腔医学院口腔颅颌面科,上海市口腔医学重点实验室, 上海市口腔医学研究所,国家口腔疾病临床研究中心,上海 200011;

收稿日期:2017-07-15 修回日期:2017-10-11 出版日期:2018-03-20 发布日期:2018-04-08
通讯作者: 朱敏,E-mail：doctorzhumin@126.com
作者简介:孙蕙珺（1991-）,女,硕士,E-mail：tina_s0327@126.com
基金资助:
上海市科学技术委员会实验动物研究项目（11140902001）

Intermittent nasal obstruction causes early effects on chondrogenesis of mandibular condylar chondrocytes

SUN Hui-jun, WANG Xiao-ling, ZHU Yan-fei, YU Qian, NIE Ping, ZHU Min

Department of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Lab of Stomatology & Shanghai Research Institute of Stomatology; National Clinical Research Center of Stomatology. Shanghai 200011, China;

Received:2017-07-15 Revised:2017-10-11 Online:2018-03-20 Published:2018-04-08

摘要/Abstract

摘要： 目的: 建立幼年大鼠双侧间歇性鼻阻塞动物模型,探讨鼻阻塞对髁突软骨细胞（mandibular condylar chondrocytes,MCCs）成软骨作用的早期影响。 方法: 64只4周龄SD大鼠随机分为4组,每组16只。A组：对照组,不做处理;B组：间歇性鼻阻塞引起开口呼吸4 d后,去除鼻阻塞因素;C组：间歇性鼻阻塞引起开口呼吸8 d后,去除鼻阻塞因素;D组：间歇性鼻阻塞引起开口呼吸16 d。鼻阻塞时间为每天8：00-16：00。在建模第4、8、12、16天,分别取4组大鼠双侧髁突,进行MCCs体外培养并鉴定,RT-PCR法检测4组MCCs成软骨标志基因（SOX9、COL2a、ACAN、PTHrp）表达的差异。采用 SPSS19.0软件包对实验数据进行统计学分析。 结果: 与对照组相比,4 d时B组、C组和D组SOX9、COL2a以及ACAN表达量均显著下降（P<0.05）,但PTHrp表达量显著升高（P<0.05）。8 d时,B组、C组和D组SOX9、COL2a、ACAN、PTHrp表达量均升高,且B组较C组和D组升高更显著（P<0.05）。12 d时,B、C和D组PTHrp的表达仍显著升高（P<0.05）,COL2a、ACAN的表达与对照组持平或降低。16 d时,C组COL2a、ACAN、PTHrp表达量较对照组显著升高（P<0.05）,B组和D组各项基因表达与对照组持平或降低。 结论: 幼年大鼠在早期双侧间歇性鼻阻塞致开口呼吸的情况下,髁突软骨细胞成软骨能力呈现先下降,随后代偿性升高,最终仍表现为下降的趋势。早期去除鼻阻塞因素后,髁突软骨细胞成软骨能力可以短期代偿性增强,但长期作用仍然是成软骨能力降低。

关键词: 鼻阻塞, 开口呼吸, 下颌骨, 髁突软骨细胞, 成软骨

Abstract: PURPOSE: In this study, we investigated the early effects of intermittent nasal obstruction on chondrogenesis of mandibular condylar chondrocytes (MCCs) via animal model. METHODS: Sixty four 4-week-old SD rats were randomly divided into 4 groups, each group contained 16 rats. Group A: control group; Group B: 4-day mouth breathing caused by nasal obstruction; Group C: 8-day mouth breathing caused by nasal obstruction; Group D: 16-day mouth breathing caused by nasal obstruction. The time of nasal obstruction was from 8:00 to 16:00 every day. Bilateral condylar MCCs were harvested and cultured in vitro, on 4th day, 8th day, 12th day and 16th day, respectively. Toluidine blue staining was used to identify MCCs,real-time polymerase chain reaction (RT-PCR) was used to detect the difference of gene expression of MCCs chondrogenic marker genes (SOX9,COL2a,ACAN,PTHrp). The data were statistically analyzed using SPSS19.0 software package. RESULTS: On 4th day of modeling, the expression of SOX9, COL2a and ACAN was lower in group B, C and D compared with group A (P<0.05); but the expression of PTHrp was higher in group B, C and D compared with group A (P<0.05). On 8th day of modeling, the expression of SOX9,COL2a,ACAN and PTHrp was higher in group B, C and D, but much higher in group B than in group C and D(P<0.05). On 12th day of modeling, group B, C and D showed a higher expression of PTHrp (P<0.05), and an equal or lower expression of COL2a, ACAN. On 16th day of modeling, the expression of COL2a, ACAN and PTHrp was increased in group C than in group A(P<0.05). CONCLUSIONS: It is suggested that during mouth breathing caused by nasal obstruction in young rats, the chondogenisis of MCCs decreased followed by a compensatory increase and finally decrease. Furthermore, early removal of nasal obstruction can cause a compensatory increase of MCCs chondrogenisis, but still causes a long-term negative influence.

Key words: Nasal obstruction, Mouth breathing, Mandibular, Mandibular condylar chondrocytes, MCCs, Chondrogenesis

中图分类号:

R782.2

孙蕙珺, 王晓玲, 朱妍菲, 于倩, 聂萍, 朱敏. 间歇性鼻阻塞对幼年大鼠髁突软骨细胞成软骨作用的早期影响[J]. 中国口腔颌面外科杂志, 2018, 16(2): 138-143.

SUN Hui-jun, WANG Xiao-ling, ZHU Yan-fei, YU Qian, NIE Ping, ZHU Min. Intermittent nasal obstruction causes early effects on chondrogenesis of mandibular condylar chondrocytes[J]. China J Oral Maxillofac Surg, 2018, 16(2): 138-143.

参考文献

[1] Rappai M, Collop N, Kemp S, et al.The nose and sleep-disordered breathing: what we know and what we do not know[J]. Chest, 2003, 124(6): 2309-2323.
[2] Rosen CL.Obstructive sleep apnea syndrome (OSAS) in children: diagnostic challenges[J]. Sleep, 1996, 19(10 Suppl): S274-277.
[3] Wong M, Siegrist M, Goodwin K.Cyclic tensile strain and cyclic hydrostatic pressure differentially regulate expression of hypertrophic markers in primary chondrocytes[J]. Bone, 2003, 33(4): 685-693.
[4] 李煌, 吴拓江, 张翔宇, 等. 体内压应力刺激下大鼠髁突软骨细胞的分离、体外培养以及差异蛋白质组学观察[J]. 实用口腔医学杂志, 2014, 30(5): 593-597
[5] 刘炯, 陈金东, 聂萍, 等. 间歇性完全鼻阻塞诱导幼年大鼠颏舌肌低氧诱导因子1α的表达[J]. 上海口腔医学, 2014, 23(2): 138-142.
[6] 刘炯, 陈金东, 朱敏, 等. 间歇性完全鼻阻塞对幼年大鼠髁突软骨细胞凋亡影响的研究[J]. 口腔医学, 2014, 34(7): 492-496.
[7] 王晓玲, 孙蕙珺, 朱妍菲, 等. 鼻阻塞对幼年大鼠髁突骨髓间充质干细胞成软骨分化的作用[J]. 中国口腔颌面外科杂志,2017, 15(1): 1-5.
[8] 朱敏, 陈金东, 聂萍, 等. 间歇性双侧完全鼻阻塞的幼年大鼠模型的建立[J]. 中华口腔正畸学杂志, 2014, 21(2): 91-94.
[9] 杨红梅, 罗颂椒, 王艳萍, 等. 大鼠下颌髁突软骨细胞体外培养研究[J]. 四川大学学报医学版, 1999, 30(1): 23-25.
[10] 朱敏. 张口呼吸的诊断与治疗[J]. 中国临床医生, 2013, 41(7): 8-9.
[11] Trabalon M, Schaal B.It takes a mouth to eat and a nose to breathe: abnormal oral respiration affects neonates' oral competence and systemic adaptation[J]. Int J Pediatr, 2012, 2012: 207605.
[12] Moss ML, Salentijn L.The primary role of functional matrices in facial growth[J]. Am J Orthod, 1969, 55(6): 566-577.
[13] Kantomaa T, Tuominen M, Pirttiniemi P.Effect of mechanical forces on chondrocyte maturation and differentiation in the mandibular condyle of the rat[J]. J Dent Res, 1994, 73(6): 1150-1156.
[14] Papachristou DJ, Papachroni KK, Papavassiliou GA, et al.Functional alterations in mechanical loading of condylar cartilage induces changes in the bony subcondylar region[J]. Arch Oral Biol, 2009, 54(11): 1035-1045.
[15] Huang L, Cai X, Li H, et al.The effects of static pressure on chondrogenic and osteogenic differentiation in condylar chondrocytes from temporomandibular joint[J]. Arch Oral Biol, 2015, 60(4): 622-630.
[16] 徐高丽, 徐婷, 汪淑华, 等. 不同静压力下髁突软骨细胞MMP-13的表达变化研究[J]. 口腔颌面外科杂志, 2016, 26(3): 156-161.
[17] 郭维华, 李松, 徐芸. 不同静压力对大鼠髁突软骨细胞生物学特性的影响[J]. 华西口腔医学杂志, 2007, 25(6): 603-605.
[18] 赵东强. 应力下髁突软骨中相关细胞因子的作用[J]. 国际口腔医学杂志, 2011, 38(4): 440-442.
[19] Chen J, Sorensen KP, Gupta T, et al.Altered functional loading causes differential effects in the subchondral bone and condylar cartilage in the temporomandibular joint from young mice[J]. Osteoarthritis Cartilage, 2009, 17(3): 354-361.
[20] Papadopoulou AK, Papachristou DJ, Chatzopoulos SA, et al.Load application induces changes in the expression levels of Sox-9, FGFR-3 and VEGF in condylar chondrocytes[J]. FEBS Lett, 2007, 581(10): 2041-2046.
[21] Huang CY, Hagar KL, Frost LE, et al.Effects of cyclic compressive loading on chondrogenesis of rabbit bone-marrow derived mesenchymal stem cells[J]. Stem Cells, 2004, 22(3): 313-323.
[22] Uekita H, Takahashi S, Domon T, et al.Changes in collagens and chondrocytes in the temporomandibular joint cartilage in growing rats fed a liquid diet[J]. Ann Anat, 2015, 202: 78-87.
[23] Rabie AB, Tang GH, Xiong H, et al.PTHrP regulates chondrocyte maturation in condylar cartilage[J]. J Dent Res, 2003, 82(8): 627-631.
[24] 刘莹, 刘艳艳, 王硕, 等. 不同下颌前伸力对髁突软骨细胞中Sox9和Ⅱ型胶原表达的影响及其意义[J]. 吉林大学学报(医学版), 2014, 40(1): 97-101.
[25] Sobue T, Yeh WC, Chhibber A, et al.Murine TMJ loading causes increased proliferation and chondrocyte maturation[J]. J Dent Res, 2011, 90(4): 512-516.
[26] Delise AM, Fischer L, Tuan RS.Cellular interactions and signaling in cartilage development[J]. Osteoarthritis Cartilage, 2000, 8(5): 309-334.

间歇性鼻阻塞对幼年大鼠髁突软骨细胞成软骨作用的早期影响

Intermittent nasal obstruction causes early effects on chondrogenesis of mandibular condylar chondrocytes

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