Fibulin-7在小鼠下颌第一磨牙发育过程中的时空表达

doi:10.19438/j.cjoms.2017.05.001

中国口腔颌面外科杂志 ›› 2017, Vol. 15 ›› Issue (5): 385-387.doi: 10.19438/j.cjoms.2017.05.001

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Fibulin-7在小鼠下颌第一磨牙发育过程中的时空表达

欧阳宁鹃，李洪亮，林雨恒，卢陈佩，代杰文, 沈国芳

上海交通大学医学院附属第九人民医院·口腔医学院口腔颅颌面科，上海市口腔医学重点实验室，上海 200011

收稿日期:2016-11-15 出版日期:2017-08-30 发布日期:2017-10-27
通讯作者: 代杰文，E-mail: daijiewen@163.com；沈国芳，E-mail：maxillofacsurg@163.com。*共同通信作者
作者简介:欧阳宁鹃（1990-），女，在读博士研究生，E-mail: oynjc0508@163.com
基金资助:
国家自然科学基金(81300842，81570947)；上海高校创新团队计划；上海高校高峰高原学科建设项目；上海市青年拔尖人才项目；上海交通大学“晨星学者”奖励计划（C类）

Spatiotemporal expression pattern of Fibulin-7 in the development process of murine mandibular first molars

OUYANG Ning-juan, LI Hong-liang, LIN Yu-heng, LU Chen-pei, DAI Jie-wen, SHEN Guo-fang.

Department of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine;
Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2016-11-15 Online:2017-08-30 Published:2017-10-27

摘要/Abstract

摘要： 目的：观察Fibulin-7蛋白在小鼠下颌第一磨牙牙胚发育过程中的时空表达特点。方法：取胚胎15.5 d、出生后第1天、第7天的C57BL/6J小鼠下颌骨标本，应用免疫组织化学方法检测Fibulin-7在下颌第一磨牙牙胚不同发育时期的表达情况。结果：Fibulin-7蛋白在小鼠下颌第一磨牙牙胚自钟状期末期至牙胚发育成熟过程中表达量逐渐增加。结论：Fibulin-7蛋白在牙胚发育的不同阶段均有表达，推测Fibulin-7参与了牙间充质细胞分化为成牙本质细胞、牙髓-牙本质复合体的形成过程。

关键词: Fibulin-7, 小鼠, 下颌第一磨牙, 时空表达

Abstract: PURPOSE: To investigate the expression patterns of Fibulin-7 in murine tooth germs of mandibular first molars at early developmental stages. METHODS: Mandible samples of C57BL/6J mice of embryonic day 15.5, postnatal day 1 and day 7 were collected. The expressions of Fibulin-7 in murine mandibular first molar germs were detected by immunohistochemistry staining. RESULTS: The expression of Fibulin-7 was detected and gradually increased from late bell stage till maturation of tooth germs in mandibular first molars. CONCLUSIONS: Fibulin-7 was expressed at different stages through the development of tooth germs, indicating their individual functions in the process of mesenchymal cells into odontoblasts and the formation of pulp-dentin complex.

Key words: Fibulin-7, Mouse, Mandibular first molars, Spatiotemporal expression patterns

中图分类号:

R782.1

欧阳宁鹃，李洪亮，林雨恒，卢陈佩，代杰文, 沈国芳. Fibulin-7在小鼠下颌第一磨牙发育过程中的时空表达[J]. 中国口腔颌面外科杂志, 2017, 15(5): 385-387.

OUYANG Ning-juan, LI Hong-liang, LIN Yu-heng, LU Chen-pei, DAI Jie-wen, SHEN Guo-fang.. Spatiotemporal expression pattern of Fibulin-7 in the development process of murine mandibular first molars[J]. China J Oral Maxillofac Surg, 2017, 15(5): 385-387.

参考文献

[1] de Vega S, Iwamoto T, Nakamura T, et al. TM14 is a new member of the fibulin family (fibulin-7) that interacts with extracellular matrix molecules and is active for cell binding [J]. J Biol Chem, 2007, 282(42): 30878-30888.
[2] Timpl R, Sasaki T, Kostka G, et al. Fibulins: a versatile family of extracellular matrix proteins[J]. Nat Rev Mol Cell Biol, 2003, 4(6): 479-489.
[3] Kobayashi N, Kostka G, Garbe JH, et al. A comparative analysis of the fibulin protein family. Biochemical characterization, binding interactions, and tissue localization[J]. J Biol Chem, 2007, 282(16): 11805-11816.
[4] Tran H, VanDusen WJ, Argraves WS. The self-association and fibronectin-binding sites of fibulin-1 map to calcium-binding epidermal growth factor-like domains [J]. J Biol Chem, 1997, 272(36): 22600-22606.
[5] Adam S, Gohring W, Wiedemann H, et al. Binding of fibulin-1 to nidogen depends on its C-terminal globular domain and a specific array of calcium-binding epidermal growth factor-like (EG) modules [J]. J Mol Biol, 1997, 272(2): 226-236.
[6] Reid KB, Day AJ. Structure-function relationships of the complement components [J]. Immunol Today, 1989, 10(6): 177-180.
[7] de Vega S, Iwamoto T, Yamada Y. Fibulins: multiple roles in matrix structures and tissue functions [J]. Cell Mol Life Sci, 2009, 66(11-12): 1890-1902.
[8] Gallagher WM, Currid CA, Whelan LC. Fibulins and cancer: friend or foe? [J]. Trends Mol Med, 2005, 11(7): 336-340.
[9] Argraves WS, Greene LM, Cooley MA, et al. Fibulins: physiological and disease perspectives[J]. EMBO Rep, 2003, 4(12): 1127-1131.
[10] Zhang HY, Timpl R, Sasaki T, et al. Fibulin-1 and fibulin-2 expression during organogenesis in the developing mouse embryo [J]. Dev Dyn, 1996, 205(3): 348-364.
[11] Miosge N, Gotz W, Sasaki T, et al. The extracellular matrix proteins fibulin-1 and fibulin-2 in the early human embryo [J]. Histochem J, 1996, 28(2): 109-116.
[12] Soemedi R, Wilson IJ, Bentham J, et al. Contribution of global rare copy-number variants to the risk of sporadic congenital heart disease [J]. Am J Hum Genet, 2012, 91(3): 489-501.
[13] Rudd MK, Keene J, Bunke B, et al. Segmental duplications mediate novel, clinically relevant chromosome rearrangements [J]. Hum Mol Genet, 2009, 18(16): 2957-2962.

Fibulin-7在小鼠下颌第一磨牙发育过程中的时空表达

Spatiotemporal expression pattern of Fibulin-7 in the development process of murine mandibular first molars

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