载有人胚胎干细胞的多孔磷酸钙骨水泥与富血小板血浆复合后修复大鼠骨缺损的实验研究

doi:10.19438/j.cjoms.2017.01.002

中国口腔颌面外科杂志 ›› 2017, Vol. 15 ›› Issue (1): 6-10.doi: 10.19438/j.cjoms.2017.01.002

载有人胚胎干细胞的多孔磷酸钙骨水泥与富血小板血浆复合后修复大鼠骨缺损的实验研究

吴珍珍, 包崇云, 李明政, 刘显

口腔疾病研究国家重点实验室,华西口腔医院,四川大学,四川成都 610041

出版日期:2017-01-20 发布日期:2017-02-27
通讯作者: 刘显,E-mail: jssyliuxian@163.com
作者简介:吴珍珍（1989-）,女,在读博士研究生,E-mail: zhenzhenwu732@126.com
基金资助:
国家自然科学基金青年基金（31400829）

Macroporous calcium phosphate cement, human embryonic stem cells and platelet-rich plasma constructs for bone regeneration in cranial defects in rats

WU Zhen-zhen, BAO Chong-yun, LI Ming-zheng, LIU Xian

State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University. Chengdu 610041, Sichuan Province, China

Online:2017-01-20 Published:2017-02-27

摘要/Abstract

摘要： 目的研究富血小板血浆（platelet rich plasma,PRP）对负载人胚胎来源的间充质干细胞（hESC-MSCs）多孔磷酸钙骨水泥（calcium phosphate cements,CPC）复合物的成骨促进作用。方法将hESC-MSCs接种于CPC表面,扫描电镜（SEM）观察hESC-MSCs在CPC表面的黏附情况。建立直径为8 mm的大鼠颅骨圆形缺损模型,将18只大鼠随机分为3组,分别是CPC + hESC-MSCs组、CPC+hESC-MSCs+30%PRP组和CPC+hESC-MSCs+50%PRP组。术后12周显微CT和H-E染色观察新骨和血管生成情况。应用SPSS16.0软件包对数据进行单因素方差分析。结果 SEM显示,细胞可良好地黏附在CPC支架的表面。显微CT示复合PRP后,CPC材料的降解程度逐渐明显,且新生骨量逐渐增加。H-E染色显示,复合PRP组的新骨生成率和血管密度高于未复合PRP组,但是复合不同浓度的PRP组间无显著差异。结论浓度为30%和50%的PRP与负载hESC-MSCs 的CPC复合后,均可促进骨缺损区新骨的生成。

关键词: 磷酸钙骨水泥, 人胚胎干细胞, 富血小板血浆, 骨组织工程

Abstract: PURPOSE: To investigate the effects of macroporous calcium phosphate cement (CPC), human embryonic stem cells (hESC) and platelet-rich plasma constructs for bone regeneration in vivo in critical-sized cranial defects in rats. METHODS: Three groups were tested in rats: (a) CPC with hESC-derived mesenchymal stem cells (CPC+hESC-MSCs); (b) CPC with hESC-MSCs and 30% platelet rich plasma (PRP) (CPC+hESC-MSCs+30%PRP) and (c) CPC+hESC-MSCs+50%PRP. 18 rats were sacrificed 12 weeks after operation. Micro-CT and bone histomorphologic analysis were conducted to compare the status of new bone formation. SPSS16.0 software package was used for one way analysis of variance. RESULTS: At 12 weeks in vivo, the groups with hESC-MSCs and PRP had more new bone formation compared with other two groups; however, there was no significant difference in new bone area fraction between different concentration of PRP groups. CONCLUSIONS: Macroporous CPC with hESC-MSCs and PRP may be useful for bone regeneration in craniofacial and orthopedic fields.

Key words: Calcium phosphate cement, Human embryonic stem cells, Platelet rich plasma, Bone tissue engineering

中图分类号:

R782.2

吴珍珍, 包崇云, 李明政, 刘显. 载有人胚胎干细胞的多孔磷酸钙骨水泥与富血小板血浆复合后修复大鼠骨缺损的实验研究[J]. 中国口腔颌面外科杂志, 2017, 15(1): 6-10.

WU Zhen-zhen, BAO Chong-yun, LI Ming-zheng, LIU Xian. Macroporous calcium phosphate cement, human embryonic stem cells and platelet-rich plasma constructs for bone regeneration in cranial defects in rats[J]. China J Oral Maxillofac Surg, 2017, 15(1): 6-10.

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载有人胚胎干细胞的多孔磷酸钙骨水泥与富血小板血浆复合后修复大鼠骨缺损的实验研究

Macroporous calcium phosphate cement, human embryonic stem cells and platelet-rich plasma constructs for bone regeneration in cranial defects in rats

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