Expression, clinical significance of cathepsin B, Beclin-1in the gingival tissues of patients with peri-implantitis

doi:10.19438/j.cjoms.2022.03.008

Abstract

Abstract: PURPOSE: To explore the changes and clinical significance of cathepsin B (Cat B) and Beclin-1 expression in the gingival tissues of patients with peri-implantitis. METHODS: One hundred and twenty-four cases with peri-implantitis were collected as the experimental group, and 124 cases of healthy gingival tissues (due to impacted tooth extraction) as the control group. The expression of Cat B and Beclin-1 mRNA and protein in the gingival tissues of the two groups were detected by RT-PCR and Western blot, respectively. One year after operation, the patients with peri-implantitis were classified into loosing/dropping group and non-loosing/dropping group. The clinical data of patients in the two groups were analyzed. Logistic multivariate regression analysis was conducted to evaluate related factors affecting implant loosing/dropping in patients with peri-implantitis. A receiver operating characteristic curve (ROC) was made to analyze the predictive value of Cat B and Beclin-1 mRNA expression in gingival tissue for the occurrence of implant loosing/dropping in patients with peri-implantitis. SPSS21.0 software package was used for data analysis. RESULTS: Cat B and Beclin-1 mRNA and protein expression in the gingival tissues of the experimental group was significantly higher than those of the control group(P<0.05). The incidence of implant loosing/dropping in patients with peri-implantitis was 16.94% one year after operation. The prevalence of diabetes, smoking history, implant loading time, procalcitonin(PCT), and the expression of Cat B and Beclin-1 mRNA in gingival tissue in the loosing/dropping group were significantly higher than those in the non-loosing/dropping group (P<0.05). Logistic multivariate analysis showed that PCT, Cat B and Beclin-1 mRNA were risk factors affecting the occurrence of implant loosing/dropping in patients with peri-implantitis (OR=2.751, 3.414, 3.196, P<0.05). ROC analysis showed that the best cut-off points for the expression of Cat B and Beclin-1 mRNA in gingival tissue to predict the occurrence of implant loosing/dropping in patients with peri-implantitis were 1.42 and 2.51, respectively, and the sensitivities were 61.90% and 76.19%, respectively; specificity was 76.70%, 60.19%, and AUC was 0.728 and 0.771, respectively. CONCLUSIONS: Compared with healthy gingiva, Cat B and Beclin-1 mRNA in the gingival tissues of patients with peri-implantitis are both highly expressed, and the expression of Cat B and Beclin-1 mRNA in the gingival tissues has an effect on peri-implant inflammation. The predictive value of the occurrence of implant loosening/dropping in patients is high, and it can be used as an important reference index for clinical evaluation of the occurrence of implant loosing/dropping.

Key words: Peri-implantitis, Cathepsin B, Beclin-1, Implant loosing, Implant dropping

CLC Number:

R782.1

XUE Qiu-bo, XUE Lu-feng, MA Cheng, DING Hao-yue. Expression, clinical significance of cathepsin B, Beclin-1in the gingival tissues of patients with peri-implantitis[J]. China Journal of Oral and Maxillofacial Surgery, 2022, 20(3): 251-256.

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