Effect of quercetin on angiogenesis and its molecular mechanism in human umbilical vein endothelial cells

doi:10.19438/j.cjoms.2018.05.001

Abstract

Abstract: PURPOSE: To investigate the effect of quercetin on angiogenesis and the biological characteristics of human umbilical vein endothelial cells (HUVECs). METHODS: HUVECs were treated with quercetin (1,5 and 10 μmol/L). CCK8 assay was used to assess the effect of quercetin on HUVECs proliferation. Wound healing assay and Matrigel-based tube formation assay were applied to observe migration and tube formation of HUVECs. Real-time PCR was used to evaluate gene expressions of VEGF and bFGF in HUVECs. Western blot was preformed to detect the change of AKT signaling pathway. Differences between groups were analyzed with SPSS 17.0 software package for ANOVA and Tukey post hoc analysis. RESULTS: Compared with the control group, quercetin significantly suppressed HUVECs proliferation, migration and tube formation in a dose-dependent manner. Gene expression of VEGF and bFGF in quercetin treated groups was significantly less than in the control group. Western blot indicated that quercetin inhibited AKT signaling pathway in HUVECs in vitro. CONCLUSION: Quercetin suppresses proliferation and angiogenesis of HUVECs in vitro, the potential mechanism of the inhibition partly involves in blockage of AKT signaling pathway.

Key words: Quercetin, HUVECs, Anti- angiogenesis, Signaling pathway

CLC Number:

GUI Zhi-peng, ZHOU Yu-ning, HU Yue, XIA Lun-guo, XU Yuan-jin. Effect of quercetin on angiogenesis and its molecular mechanism in human umbilical vein endothelial cells[J]. China Journal of Oral and Maxillofacial Surgery, 2018, 16(5): 385-390.

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