Abstract: PURPOSE: To investigate the function of miR-582-5p in cell invasion and migration in salivary gland adenoid cystic carcinoma(ACC). METHODS: The expression levels of miR-582-5p were detected by real-time PCR in parotid ACC cancers and normal tissues. Cell invasion and migration were detected by Transwell assays. Western blotting and IHC were performed to detect the expression of FOXC1, which was predicted as target gene of miR-582-5p. Student's t test or one-way ANOVA were performed to analyze the data using SPSS 16.0 software package. RESULTS: After transfection with miR-582-5p mimics, the expression of miR-582-5p was significantly up-regulated（P<0.001）. The invasion and migration ability of SACC-83 was substantially inhibited by miR-582-5p.The target gene of miRNA was identified by luciferase reporter assay. Western blotting showed that FOXC1 was positive in SACC-83. With the elevated expression of miR-582-5p, the expression of FOXC1 was down regulated substantially in SACC-83. FOXC1 was found with higher expression in SACC compared with adjacent normal tissues. CONCLUSIONS: Lower expression of miR-582-5p played an important role in the process of invasion and migration of SACC-83. Overexpression of miR-582-5p in SACC-83 could substantially suppress the ability of cell invasion and migration. MiR-582-5p may exert its action by regulating the expression of target gene FOXC1.