口服硝酸盐对大鼠背部随意皮瓣的保护作用及机制探讨

doi:10.19438/j.cjoms.2021.02.002

中国口腔颌面外科杂志 ›› 2021, Vol. 19 ›› Issue (2): 105-109.doi: 10.19438/j.cjoms.2021.02.002

口服硝酸盐对大鼠背部随意皮瓣的保护作用及机制探讨

庞宝兴¹, 卜令学¹, 贾暮云¹, 袁荣涛², 尚伟¹

1.青岛大学附属医院口腔颌面外科,山东青岛 266003;
2.青岛市市立医院口腔医学中心,山东青岛 266071

收稿日期:2020-04-07 修回日期:2020-09-25 出版日期:2021-03-20 发布日期:2021-05-11
通讯作者: 庞宝兴,E-mail：pangbx@qduhospital.cn
作者简介:庞宝兴(1982-),男,博士,主治医师
基金资助:
山东省自然科学基金（ZR2017BH034）

The effect of dietary nitrate on rat dorsal random flap and its mechanism

PANG Bao-xing¹, BU Ling-xue¹, JIA Mu-yun¹, YUAN Rong-tao², SHANG Wei¹

1. Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Qingdao University, Qingdao University. Qingdao 266003;
2. Qingdao Municipal Hospital, Affiliated to Shandong University. Qingdao 266071, Shandong Province, China

Received:2020-04-07 Revised:2020-09-25 Online:2021-03-20 Published:2021-05-11

摘要/Abstract

摘要： 目的探讨口服硝酸盐对大鼠背部随意皮瓣的影响,以硝酸盐-亚硝酸盐-NO通路为切入点,探讨其可能的机制。方法将24只雄性Wistar大鼠随机分为硝酸盐组、氯化钠组和对照组,每组8只。采用改良大鼠背部随意皮瓣制作方法造模。硝酸盐干预组在术前7 d及术后每天口服0.5 mmol/L硝酸钠,氯化钠组每天口服等量氯化钠,对照组每天口服蒸馏水。术后第7天,检测各组皮瓣的存活情况,大鼠血清中硝酸盐、亚硝酸盐、肿瘤坏死因子（tumor necrosis factor alpha,TNF-a）和白介素6（interleukin-6,IL-6）水平,以及皮瓣组织中超氧化物歧化酶（superoxide dismutase,SOD）和丙二醛（malondialdehyde,MDA）水平。采用SPSS 20.0软件包对数据进行t检验。结果硝酸盐组皮瓣的存活面积显著高于对照组（P<0.05）。H-E染色显示,硝酸盐明显减轻了皮瓣的组织学损伤。硝酸钠显著增加了血清中硝酸盐和亚硝酸盐水平（P<0.05）,并显著下调血清中TNF-a和IL-6水平（P<0.05）。此外,硝酸盐组皮瓣组织内MDA表达显著减少（P<0.05）,SOD表达显著增加（P<0.05）。结论口服硝酸盐可通过调控皮瓣的氧化应激和炎症反应保护皮瓣。

关键词: 硝酸盐, 皮瓣, 一氧化氮, 炎症反应, 氧化应激

Abstract: PURPOSE: To investigate the effect of oral nitrate on the survival of rat dorsal random flap, and to explore its potential mechanism by taking nitrate-nitrite-NO pathway as the breakthrough point. METHODS: Twenty-four healthy adult male Wistar rats were randomly divided into 3 groups (n=8). Modified McFarlane rat dorsal random skin flap was used to make the model. The experimental group was given 0.5 mmol/L sodium nitrate 7 days before operation and daily after operation, while the control group was given the same amount of sodium chloride daily, and the blank group was given distilled water daily. The survival area of the flap was observed on the 7th postoperative day. On the 7th day after operation, the contents of serum nitrate and nitrite in rats were detected, and the levels of TNF-α and IL-6 in serum were further detected. Meanwhile, histological changes of skin flap were observed and the levels of malondialdehyde(MDA) and superoxide dismutase(SOD) were analyzed in flap tissue samples. The data were analyzed with SPSS software package. RESULTS: The survival area of the flap in the experimental group was significantly higher than that in the control group on the 7th day after operation, and the serum nitrate and nitrite contents were significantly higher than those in the control group. At the same time, H-E staining found that nitrate reduced the histological damage of the flap. Through observation of the expression of serum TNF-α and IL-6, it was found that nitrate reduced inflammatory reaction. In addition, the oxidative stress marker MDA was reduced, while the activity of SOD was restored after nitrate treatment. CONCLUTIONS: Oral nitrate can improve the survival area of random flap in rats. The mechanism is associated by promoting angiogenesis and reducing circulating inflammatory factors through nitrate-nitrite - NO pathway.

Key words: Nitrate, Skin flap, Nitric oxide, Inflammation, Oxidative stress

中图分类号:

R782.2

庞宝兴, 卜令学, 贾暮云, 袁荣涛, 尚伟. 口服硝酸盐对大鼠背部随意皮瓣的保护作用及机制探讨[J]. 中国口腔颌面外科杂志, 2021, 19(2): 105-109.

PANG Bao-xing, BU Ling-xue, JIA Mu-yun, YUAN Rong-tao, SHANG Wei. The effect of dietary nitrate on rat dorsal random flap and its mechanism[J]. China J Oral Maxillofac Surg, 2021, 19(2): 105-109.

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口服硝酸盐对大鼠背部随意皮瓣的保护作用及机制探讨

The effect of dietary nitrate on rat dorsal random flap and its mechanism

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