自体浓缩生长因子膜对山羊髁突软骨缺损的修复作用

中国口腔颌面外科杂志 ›› 2014, Vol. 12 ›› Issue (3): 205-209.

自体浓缩生长因子膜对山羊髁突软骨缺损的修复作用

孙玉环¹,何冬梅¹,杨驰¹,孙宁宁¹,蔡怡华¹,王丽珍²,张萍³

1.上海交通大学医学院附属第九人民医院·口腔医学院口腔外科,2.口腔病理科,3.上海市口腔医学重点实验室,上海 200011

收稿日期:2013-07-31 出版日期:2014-05-10 发布日期:2014-07-15
通讯作者: 何冬梅,E-mail:lucyhe119@163.com E-mail:huanhuanishappy@163.com
作者简介:孙玉环(1987-),女,硕士研究生
基金资助:
上海市科学技术委员会资助项目(08DZ2271100,13XD1402300);上海高校创新团队发展计划;教育部留学回国人员科研启动基金;卫生行业公益基金(201002017);上海市自然科学基金(13ZR1423500)

Effect of concentrated growth factors on goat condylar cartilage defect

SUN Yu-huan¹, HE Dong-mei¹, YANG Chi¹,SUN Ning-ning¹, CAI Yi-hua¹, WANG Li-zhen², ZHANG Ping³

1.Department of Oral Surgery; 2.Department of Oral Pathology, Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; 3.Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2013-07-31 Online:2014-05-10 Published:2014-07-15

摘要/Abstract

摘要： 目的:观察自体浓缩生长因子膜(concentrated growth factors,CGF)对山羊髁突软骨缺损的修复作用。方法:6个月龄山羊3只,每只切除双侧髁突表面软骨,抽取自体静脉血,离心后制作CGF膜,覆盖一侧髁突表面,另外一侧作为对照。术后1个月处死动物并取材,进行大体标本和组织学观察。结果:术后1个月,对照侧髁突表面凹凸不平,实验侧髁突表面光滑,有类软骨样组织覆盖。H-E染色显示,对照侧髁突表面软骨下骨裸露,骨吸收活跃,有破骨细胞;炎症反应明显,血管扩张;实验侧髁突未见明显炎症反应和骨吸收,骨表面为纤维组织覆盖。结论:CGF可减少软骨损伤后的炎症反应和骨吸收,促进纤维组织形成,对软骨下骨有一定的保护作用。

关键词: 浓缩生长因子, 髁突, 软骨损伤, 软骨修复

Abstract: PURPOSE: To investigate the therapeutic effect of concentrated growth factors (CGF) on the healing process of goat condylar cartilage defect. METHODS: Three goats at the age of 6 months (average weight=16 kg) were used in this study. Each animal had bilateral condyle cartilage resected, then autologous venous blood was collected to obtain CGF. CGF was used to cover one side of the condyle cartilage defect, the other side of the condyle cartilage defect was uncovered as control. Gross observation and histological examination were performed 1 month after operation. RESULTS: The condylar surface of the control side was uneven while the experimental condylar surface was smooth, covered by cartilage-like tissues. H-E staining of the control side showed exposed subchondral bone, active bone resorption by osteoclasts and inflammatory reaction. While the control side showed no obvious inflammatory reaction and bone resorption. The bone surface was covered by fibrous tissues. CONCLUSION: CGF can reduce inflammation and bone resorption after cartilage damage. It can also promote fibrous tissue repair to protect the subchondral bone.Supported by Research Fund of Science and Technology Committee of Shanghai Municipality (08DZ2271100, 13XD1402300), Program for Innovative Research Team of Shanghai Municipal Education Commission, Scientific Research Foundation of Ministry of Education for Returned Overseas Scholars, Research Fund of the Ministry of Health (201002017), and Natural Science Foundation of Shanghai Municipality (13ZR1423500).

Key words: Concentrated growth factors, Condyle, Cartilage defect, Cartilage repair

中图分类号:

R782.6

孙玉环,何冬梅,杨驰,孙宁宁,蔡怡华,王丽珍,张萍. 自体浓缩生长因子膜对山羊髁突软骨缺损的修复作用[J]. 中国口腔颌面外科杂志, 2014, 12(3): 205-209.

SUN Yu-huan, HE Dong-mei, YANG Chi,SUN Ning-ning, CAI Yi-hua, WANG Li-zhen, ZHANG Ping. Effect of concentrated growth factors on goat condylar cartilage defect[J]. China J Oral Maxillofac Surg, 2014, 12(3): 205-209.

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