山羊颞下颌关节损伤模型的建立及组织学评价

中国口腔颌面外科杂志 ›› 2014, Vol. 12 ›› Issue (1): 13-19.

山羊颞下颌关节损伤模型的建立及组织学评价

李凌志^{1, *}, 王丽珍^{2, *}, 何冬梅^{1, #}, 杨驰^{1, #}

1.上海交通大学医学院附属第九人民医院·口腔医学院口腔外科, 上海市口腔医学重点实验室,上海 200011; 2.口腔病理科,上海市口腔医学重点实验室,上海 200011

收稿日期:2012-12-19 修回日期:2013-08-01 出版日期:2014-02-10 发布日期:2014-02-10
通讯作者: 何冬梅,E-mail: lucyhe119@163.com；杨驰,Tel：021-23271699-5218,E-mail: yangchi63@hotmail.com。#并列通信作者
作者简介:李凌志(1980-),男,博士,医师,E-mail:lexusli80@gmail.com；王丽珍(1969-),女,硕士,主治医师,E-mail:linzhenwang535@hotmail.com。*并列第一作者
基金资助:
国家自然科学基金(81070848); 国家留学基金委员会回国人员科研启动基金; 上海市科学技术委员会资助项目(08DZ2271100); 上海高校创新团队发展计划

Establishment and histological evaluation of traumatic temporomandibular joint model in goat

LI Ling-zhi¹, WANG Li-zhen², HE Dong-mei¹, YANG Chi¹

1.Department of Oral Surgery, College of Stomatology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China; 2.Department of Oral Pathology, College of Stomatology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2012-12-19 Revised:2013-08-01 Online:2014-02-10 Published:2014-02-10
Supported by:
Supported by National Natural Science Foundation of China (81070848), Research Fund of Science and Technology Committee of Shanghai Municipality (08DZ2271100), and Program for Innovative Research Team of Shanghai Municipal Education Commission.

摘要/Abstract

摘要： 目的: 建立山羊颞下颌关节损伤模型,比较不同损伤类型的组织病理学改变及对预后的影响。方法：模拟临床损伤类型,16只成年山羊双侧关节随机分为4组。DD组：剪断关节盘后附着,牵引向前,固定于冠突根部,形成不可复性关节盘前移位；CCD组：磨除髁突外侧1/3软骨,造成髁突软骨损伤；DD+CCD组：形成关节盘前移位+髁突软骨损伤。C组：空白对照组。分别于术后2周和1个月观察3种损伤类型的大体及组织学改变。对髁突软骨进行组织学评分,采用SPSS17.0软件包比较4组之间的差异。结果：DD+CCD组,2周和1个月时见囊内纤维性黏连,剩余软骨粗糙；镜下见剩余软骨结构紊乱,蛋白多糖降解。1个月时,软骨剥脱及囊内纤维黏连加重。DD组,2周和1个月时剩余髁突软骨结构紊乱,纤维带完整,少量血管再生；蛋白多糖少量降解。CCD组,2周和1个月时缺损区与关节盘发生黏连；剩余软骨结构和蛋白多糖与空白对照组相比基本正常。各组之间比较,软骨退行性变程度1个月时比2周时更严重,统计评分按严重程度分别为DD+CCD组(11.75±0.75)、DD组(8.75±0.25)、CCD组(1.75±0.85)和C组(0.00±0.00),各组之间比较均存在显著差异(P<0.05)。结论：通过手术成功建立了3种山羊TMJ损伤模型,其中,DD+CCD组对关节软骨的破坏最严重,DD次之,CCD最轻。

关键词: 颞下颌关节, 关节盘前移位, 髁突软骨损伤, 创伤性骨关节炎, 动物模型

Abstract: PURPOSE: To establish traumatic temporomandibular joint model in goat, and evaluate the histopathological changes. METHODS: According to the clinical traumatic type, 16 adult goats’ bilateral joints were randomly divided into 4 groups following surgical procedures. Disc displacement without reduction (DD): the attachment of disc was severed, and pulled anteriorly; the disc was fixed in the root of coronoid process. Condylar cartilage defect (CCD): the inferior compartment of TMJ was open to grind off lateral 1/3 condylar cartilage. Disc displacement and condylar cartilage defect (DD+CCD): the above two METHODS were used. Blank control group(C): nothing was done in TMJ. The TMJs were removed and observed 2 weeks and 1 month after surgery. The histopathological score of condylar cartilage was observed and was statistically analysed between each two groups (4 groups) with SPSS 17.0 software package. RESULTS: DD+CCD showed fibrous adhesion and coarse condylar cartilage; histologically, the composition of condylar cartilage was in disorder; and matrix proteoglycan decreased at 2-week and 1-month. Partial cartilage desquamation and fibrous adhesion exacerbated at 1-month. DD showed disordered cartilage composition, intact superfacial zone, little angiogenesis and proteoglycan degradation at 2-week and 1-month. CCD showed fibrosis between disc and defective cartilage in defective condyle, and the remnant cartilage composition and proteoglycan were normal at 2-week and 1-month. Cartilage degeneration was more serious at 1-month than 2-week in each group; and cartilage score was significantly different (P<0.05)in the following order: DD+CCD(11.75±0.75),DD(8.75±0.25),CCD(1.75±0.85),and C(0.00±0.00). CONCLUSION: Three traumatic TMJ models in goat were successfully established. The degeneration of cartilage in DD+CCD was most severe, and CCD was the mildest.

Key words: Temporomandibular joint, Disc displacement, Condylar cartilage defect, Traumatic osteoarthritis, Animal model

李凌志, 王丽珍, 何冬梅, 杨驰. 山羊颞下颌关节损伤模型的建立及组织学评价[J]. 中国口腔颌面外科杂志, 2014, 12(1): 13-19.

LI Ling-zhi, WANG Li-zhen, HE Dong-mei, YANG Chi. Establishment and histological evaluation of traumatic temporomandibular joint model in goat[J]. China J Oral Maxillofac Surg, 2014, 12(1): 13-19.

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