China Journal of Oral and Maxillofacial Surgery ›› 2022, Vol. 20 ›› Issue (4): 328-333.doi: 10.19438/j.cjoms.2022.04.003

• Original Articles • Previous Articles     Next Articles

Establishment and identification of patient-derived organoid model in head and neck squamous carcinoma

WANG Chong, KANG Xin-dan, WANG Rui, WU Ya-ping, SUN Lu-lu, ZHANG Zhi-yuan, SUN Shu-yang   

  1. Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011,China
  • Received:2021-12-21 Revised:2022-04-17 Online:2022-07-20 Published:2022-08-02

Abstract: PURPOSE: To construct and identify patient-derived organoid (PDO) model of head and neck squamous cell carcinoma(HNSCC). METHODS: Tumor cells, isolated from HNSCC samples, were cultured and passaged in matrix gel with three dimensions. The growth was also recorded at regular intervals. The correlation between patient tumor tissue and PDO models in histopathological and tumor molecular markers was compared using H-E and immunohistochemical staining assay. RESULTS: This study displayed 3 cases of PDO models that could be passaged stably, and the histopathology and tumor marker identification results showed that the model and the patient's tissues were consistent with each other. CONCLUSIONS: The PDO model constructed from samples of HNSCC could reflect the patient's histomorphology and biomarker characteristics, and could be utilized as a reliable preclinical model in the further HNSCC study.

Key words: Head and neck squamous cell carcinoma, Patient-derived organoid, Preclinical model

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