China Journal of Oral and Maxillofacial Surgery ›› 2022, Vol. 20 ›› Issue (4): 320-327.doi: 10.19438/j.cjoms.2022.04.002

• Original Articles • Previous Articles     Next Articles

Study on the mechanism of miR-330-3p in cartilage degeneration in temporomandibular joint osteoarthritis in rats

WANG Chu-yao1,2, ZOU Lu-xiang1, LU Chuan1, HE Dong-mei1   

  1. 1. Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011;
    2. School of Stomatology, Qingdao University. Qingdao 266000, Shandong Province, China
  • Received:2022-04-25 Revised:2022-06-01 Online:2022-07-20 Published:2022-08-02

Abstract: PURPOSE: To study the mechanism of miR-330-3p in cartilage degeneration in temporomandibular joint osteoarthritis(TMJOA) in rats. METHODS: Human chondrocytes SW1353 was stimulated by inflammation and stress respectively and rat TMJOA model was established through unilateral anterior crossbite(UAC). The expression of miRNA-330-3p in vitro and in vivo was detected by RT-qPCR. The proliferation and metabolism changes of SW1353 were detected by EDU fluorescence staining and immunoblotting after miRNA-330-3p overexpression or inhibition. Furthermore, bioinformatics analysis was performed to predict and screen the downstream targets of miRNA-330-3p. RT-qPCR and immunoblotting were used to clarify the regulation of miRNA-330-3p on the target and the function of target. After intra-articular injection of miRNA-330-3p in TMJOA rats, the changes in cartilage metabolism were detected by immunohistochemistry to clarify the effect of miRNA-330-3p on TMJOA. SPSS 22.0 software package was used to analyze the data. RESULTS: The expression of miRNA-330-3p decreased in chondrocytes under stress or inflammatory stimuli in vitro and in TMJOA rats. Inhibition of miRNA-330-3p decreased chondrocyte proliferation, reduced SOX9 and increased MMP13 expression, while overexpression of miRNA-330-3p had opposite effects. Bioinformatics analysis revealed that CTNNB1 was one of the possible downstream targets, and its expression was elevated after inflammatory/stress stimuli in vitro and in TMJOA rats. SOX9 expression decreased, and MMP13 expression increased after overexpression of CTNNB1, with opposite results for inhibition of CTNNB1. There was a negative relationship between miRNA-330-3p and CTNNB1. Intra-articular injection of miRNA-330-3p in TMJOA rats alleviated cartilage degeneration. CONCLUSIONS: miRNA-330-3p attenuates cartilage degeneration in TMJOA by inhibiting the expression of CTNNB1.

Key words: Temporomandibular joint osteoarthritis, Cartilage degeneration, miR-330-3p, CTNNB1

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