China Journal of Oral and Maxillofacial Surgery ›› 2022, Vol. 20 ›› Issue (2): 123-128.doi: 10.19438/j.cjoms.2022.02.004

• Original Articles • Previous Articles     Next Articles

Effect of shRNA interference Rce1gene expression on the radiosensitivity of oral squamous cell carcinoma cells

SUN Da-wei1, ZHANG Yun2, ZHU Yong2   

  1. 1. Department of Stomatology,2. Department of Radiotherapy, Baoji Central Hospital. Baoji 721008, Shaanxi Province, China
  • Received:2021-09-10 Revised:2021-12-10 Online:2022-03-20 Published:2022-03-20

Abstract: PURPOSE: To observe the effect of shRNA interference with Ras convertase 1 (Rce1) gene expression on the radiosensitivity of oral squamous cell carcinoma (OSCC) cells. METHODS: CAL27 cells in log phase were taken and randomly divided into control group, shRNA-NC group and shRNA-Rce1 group. The control group was not treated, while shRNA-NC group and shRNA-Rce1 group were transfected with shRNA-NC and shRNA-Rce1 expression vector by liposome transfection method, respectively. Dimethylthiazole (MTT) method was used to detect the proliferation inhibition rate of different radiation doses (2, 4, 6, 8, 10 Gy) on CAL27 cells, and the half-dose radiation inhibitory amount on the cells was calculated. AnnexinV-FITC/PI double staining was used to detect the apoptosis rate. Western blot was used to detect the expression levels of cellular B-cell lymphoma-2(Bcl-2), Survivin and Caspase-3 (Caspase-3) protein. SPSS 19.0 software package was used for data analysis. RESULTS: Compared with the control group and shRNA-NC group, the inhibition rate of different radiation doses on CAL27 cell proliferation in the shRNA-Rce1 group were significantly increased, while the half inhibition dose was significantly decreased(P<0.05). Compared with the control group and shRNA-NC group, the apoptotic rate in the shRNA-Rce1 group was significantly increased (P<0.05). Compared with the control group and shRNA-NC group, the expression of Bcl-2 and Survivin protein in the shRNA-Rce1 group was significantly decreased, while the expression of Caspase-3 protein was significantly increased (P<0.05). CONCLUSIONS: shRNA interference with Rce1 gene expression can enhance the radiosensitivity of OSCC cells, reduce half inhibition dose, promote cell apoptosis, reduce the expression of apoptosis-inhibiting proteins Bcl-2 and Survivin, and increase the expression of apoptotic protein Caspase-3.

Key words: Oral squamous cell carcinoma, Ras converting enzyme 1, Radiosensitivity

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