China Journal of Oral and Maxillofacial Surgery ›› 2021, Vol. 19 ›› Issue (6): 562-567.doi: 10.19438/j.cjoms.2021.06.015

• Review Articles • Previous Articles     Next Articles

Application of lineage tracing technique based on Cre/Loxp recombinase system in the study of osteogenic remodeling

XU Ning-wei1, WANG Jing1, GONG Jia-jing1, ZHANG Jin1, JIN An-ting2, JIANG Ling-yong2   

  1. 1. College of Stomatology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China;
    2. Department of Oral and Maxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China
  • Online:2021-12-25 Published:2022-03-02

Abstract: [Summary] Cell lineage tracing technology is an important tool to study the characteristics of stem cells, among which, the one based on Cre/Loxp recombinase system is a great breakthrough. General recombinase system realizes the tissue specificity of reporter gene, based on which, inducible recombinase system makes controlling the expression time of reporter gene possible. The study of the mechanism of osteogenic remodeling is related to the fate of osteoblast lines, which is of great significance to the study of bone remodeling and the application of tissue engineering. This paper reviewed the application of lineage tracing technique based on Cre/Loxp recombinase system in the study of the origin, differentiation, lineage and hierarchy of osteoblast lines and their function in development and repair, and discussed its scope of application, advantages and disadvantages.

Key words: Lineage tracing, Osteogenic remodeling, Cre/Loxp recombinase system

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