Study on the osteogenic potential of macrophages in alveolar cleft repaired with β-TCP scaffold

doi:10.19438/j.cjoms.2021.04.005

Abstract

Abstract: PRUPOSE: To study the osteogenic potential of 3D printed β-TCP scaffold combined with bone marrow stem cells（BMSCs） repairing alveolar cleft and the role of macrophages in osteogenesis. METHODS: Bilateral maxillary first molars were extracted in the first eight rats to form a box shaped bone defect in the size of 4 mm × 4 mm × 3 mm. 3D printed β-TCP scaffold with BMSCs was implanted on one side and control scaffold was implanted on the other side eight weeks later. In the second group, 3D printed β-TCP scaffold with BMSC was placed on both sides at the same time, and macrophages were removed on one side. Eight weeks after operation, Micro-CT scanning and histological analysis were performed to detect bone formation. SAS 8.0 software was performed for statistical analysis. RESULTS: BV/TV ratio was (41.38±3.334)% in β-TCP group and (30.42±2.964)% in the control group (P<0.05). BV/TV ratio was (18.71±2.193)% in macrophage-depletion group and (39.81%±2.678)% in the control group. There was no significant difference between the two groups with the same β-TCP side. CONCLUSIONS: BMSCs combined with 3D printed β-TCP scaffold has satisfying osteogenic ability, and macrophages play an important role in β-TCP mediated osteogenesis. The depletion of macrophages hinders new bone formation.

Key words: β-tricalcium phosphate, Alveolar cleft, Osteoimmunology, Bone substitute

CLC Number:

R782.2

SHAN Yu-hua, YING Xi-yu, ZHANG Xiao-yu, CHEN Zhen-qi. Study on the osteogenic potential of macrophages in alveolar cleft repaired with β-TCP scaffold[J]. China Journal of Oral and Maxillofacial Surgery, 2021, 19(4): 315-319.

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