Application of PEGS/β-TCP membrane in promoting bone regeneration of rat calvarial defect

doi:10.19438/j.cjoms.2020.03.006

Abstract

Abstract: PURPOSE: The purpose of this study was to explore the effect of PEGS/β-TCP membrane on guided bone regeneration. METHODS: PEGS/β-TCP membrane was co-cultured with rat bone marrow mesenchymal stem cells (rBMSCs) to observe cell adhesion, proliferation and osteogenic differentiation. Animal model of rat skull defect was established, and PEGS/β-TCP membrane was implanted in the area of the defect. Micro-CT was used to observe the formation of new bone in bone defect area, and H-E staining of decalcified tissue was used to observe the degree of inflammatory infiltration and new bone formation. Statistical analysis was performed with SPSS 22.0 software package. RESULTS: rBMSCs can be well adhered and widely distributed on PEGS/β-TCP membrane. Quantitative analysis of dsDNA at 1, 4 and 7 days showed that the DNA content in both groups increased from day 1 to day 7, and there was significant difference between the control group and the experimental group at day 7 (P<0.001). Alkaline phosphatase (ALP) activity of rBMSCs on PEGS/β-TCP membrane increased significantly after osteogenic induction in vitro. ALP activity of the experimental group was significantly higher than that of the control group (P<0.01). The results of Alizarin red mineralization staining and ALP staining showed the same trend at day 21. Micro-CT examination and H-E staining showed that the formation of new bone in the experimental group was significantly higher than that in the control group, and the inflammatory reaction in each group was mild. CONCLUSIONS: This study shows that PEGS/β-TCP composite membrane had ideal cytocompatibility and guided bone regeneration effect, which provides theoretical support for further study and application of PEGS/β-TCP membrane in guided bone regeneration.

Key words: Guided bone regeneration, PEGS/β-TCP membrane, Mesenchymal stem cells, Osteogenic differentiation, Rat, Calvarial defect

CLC Number:

R782.2

ZHI Yin, YU Shuang, SHEN Hong-zhou, SI Jia-wen, YUAN Yuan, SHI Jun, LIU Chang-sheng. Application of PEGS/β-TCP membrane in promoting bone regeneration of rat calvarial defect[J]. China Journal of Oral and Maxillofacial Surgery, 2020, 18(3): 219-225.

References

[1] Sharif F, Ur Rehman I, Muhammad N, et al.Dental materials for cleft palate repair[J]. Mater Sci Eng C Mater Biol Appl, 2016, 61: 1018-1028.
[2] 徐丽娜, 王琛. GBR技术与种植相关的研究进展[J]. 口腔医学, 2017, 37(9): 829-832.
[3] Retzepi M, Donos N.Guided bone regeneration: biological principle and therapeutic applications[J]. Clin Oral Implants Res, 2010, 21(6): 567-576.
[4] Sanz M, Vignoletti F.Key aspects on the use of bone substitutes for bone regeneration of edentulous ridges[J]. Dent Mater, 2015, 31(6): 640-647.
[5] Wang J, Wang L, Zhou Z, et al.Biodegradable polymer membranes applied in guided bone/tissue regeneration: a review[J]. Polymers (Basel), 2016, 8(4): E115.
[6] Friedmann A, Dehnhardt J, Kleber BM, et al.Cytobiocompatibility of collagen and ePTFE membranes on osteoblast-like cells in vitro[J]. J Biomed Mater Res A, 2008, 86(4): 935-941.
[7] McGovern JA, Griffin M, Hutmacher DW. Animal models for bone tissue engineering and modelling disease[J]. Dis Model Mech, 2018, 11(4): dmm033084.
[8] Elgali I, Omar O, Dahlin C, et al.Guided bone regeneration: materials and biological mechanisms revisited[J]. Eur J Oral Sci, 2017, 125(5): 315-337.
[9] 沈鑫,刘雪,宿峰, 等. 完全可降解聚乳酸及其共聚物的生物相容性:研究、应用与未来[J]. 中国组织工程研究, 2018, 22(14): 2259-2264.
[10] Jung RE, Kokovic V, Jurisic M, et al.Guided bone regeneration with a synthetic biodegradable membrane: a comparative study in dogs[J]. Clin Oral Implants Res, 2011, 22(8): 802-807.
[11] Ho MH, Chang HC, Chang YC, et al.PDGF-metronidazole-encapsulated nanofibrous functional layers on collagen membrane promote alveolar ridge regeneration[J]. Int J Nanomedicine, 2017, 12: 5525-5535.
[12] De Santis R, Russo A, Gloria A, et al.Towards the design of 3D fiber-deposited poly(epsilon-caprolactone)/iron-doped hydroxyapatite nanocomposite magnetic scaffolds for bone regeneration[J]. J Biomed Nanotechnol, 2015, 11(7): 1236-1246.
[13] Yang F, Both SK, Yang X, et al.Development of an electrospun nano-apatite/PCL composite membrane for GTR/GBR application[J]. Acta Biomater, 2009, 5(9): 3295-3304.
[14] 吕杰, 程静, 侯晓蓓. 生物医用材料导论[M]. 上海: 同济大学出版社, 2016: 166.
[15] Blázquez R, Sánchez-Margallo FM, Álvarez V, et al.Surgical meshes coated with mesenchymal stem cells provide an anti-inflammatory environment by a M2 macrophage polarization[J]. Acta Biomater, 2016, 31: 221-230.
[16] Caballé-Serrano J, Munar-Frau A, Ortiz-Puigpelat O, et al.On the search of the ideal barrier membrane for guided bone regeneration[J]. J Clin Exp Dent, 2018, 10(5): e477-e483.
[17] Elgali I, Turri A, Xia W, et al.Guided bone regeneration using resorbable membrane and different bone substitutes: Early histological and molecular events[J]. Acta Biomater, 2016, 29: 409-423.
[18] Dalby MJ, Gadegaard N, Oreffo RO.Harnessing nanotopography and integrin-matrix interactions to influence stem cell fate[J]. Nat Mater, 2014, 13(6): 558-569.
[19] Ma Y, Zhang W, Wang Z, et al.PEGylated poly(glycerol sebacate)-modified calcium phosphate scaffolds with desirable mechanical behavior and enhanced osteogenic capacity[J]. Acta Biomater, 2016, 44: 110-124.
[20] Masoudi Rad M, Nouri Khorasani S, Ghasemi-Mobarakeh L, et al.Fabrication and characterization of two-layered nanofibrous membrane for guided bone and tissue regeneration application[J]. Mater Sci Eng C Mater Biol Appl, 2017, 80: 75-87.
[21] Wassell RW, Walls AW, Steele JG. Crowns and extra-coronal restorations: materials selection[J]. Br Dent J, 2002, 192(4): 199-202, 205-211.
[22] Tevlek A, Hosseinian P, Ogutcu C, et al.Bi-layered constructs of poly(glycerol-sebacate)-beta-tricalcium phosphate for bone-soft tissue interface applications[J]. Mater Sci Eng C Mater Biol Appl, 2017, 72: 316-324.
[23] Chen G, Dong C, Yang L, et al.3D Scaffolds with different stiffness but the same microstructure for bone tissue engineering[J]. ACS Appl Mater Interfaces, 2015, 7(29): 15790-15802.
[24] Chen Z, Chen L, Liu R, et al.The osteoimmunomodulatory property of a barrier collagen membrane and its manipulation via coating nanometer-sized bioactive glass to improve guided bone regeneration[J]. Biomater Sci, 2018, 6(5): 1007-1019.
[25] Gentile P, Ferreira AM, Callaghan JT, et al.Multilayer nanoscale encapsulation of biofunctional peptides to enhance bone tissue regeneration in vivo[J]. Adv Healthc Mater, 2017, 6(8): 1601182.