Establishment of a new cell line of oral squamous cell carcinoma cell: TSCC2016

doi:10.19438/j.cjoms.2019.03.003

Abstract

Abstract: PURPOSE: To establish a new cell line of oral squamous cell carcinoma（OSCC）, and explore its biological characteristics. METHODS: Fresh tumor tissue from a female patient, which was pathologically confirmed to be oral squamous cell carcinoma (OSCC), was collected during surgery. Tissue sample was cut into small pieces and cultured in nutrient solution. A new cell line of oral squamous cell carcinoma (TSCC2016) was established, and its cellular morphology was described. Cell proliferation, epithelial cell differentiation, tumorigenicity, and cell karyotype were examined. The data were analyzed with SPSS13.0 software package. RESULTS: TSCC2016 was successfully established and subcultured for more than 100 passages. TSCC2016 cells had characteristics of polygonal epithelial cell, epithelial cell differentiation, and good tumorigenicity. STR analysis showed that TSCC2016 cells were primarily cultured OSCC cells without any other cell line contamination. CONCLUSIONS: The new cell line of OSCC cell line (TSCC2016) is a stable cell line for further basic research.

Key words: Oral squamous cell carcinoma, Cell line, Primary culture, Cell culture

CLC Number:

R739.8

ZHU Dong-wang, SUN Wen-wen, ZHAO Tong-chao, ZHONG Lai-ping, ZHANG Chen-ping, ZHANG Zhi-yuan. Establishment of a new cell line of oral squamous cell carcinoma cell: TSCC2016[J]. China Journal of Oral and Maxillofacial Surgery, 2019, 17(3): 204-209.

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