水通道蛋白3在口腔鳞癌中的表达及临床意义

doi:10.19438/j.cjoms.2022.05.011

中国口腔颌面外科杂志 ›› 2022, Vol. 20 ›› Issue (5): 478-482.doi: 10.19438/j.cjoms.2022.05.011

水通道蛋白3在口腔鳞癌中的表达及临床意义

许刚¹, 宋其义², 蒋卫东¹, 刘景秀³

1.盘锦辽油宝石花医院口腔科,辽宁盘锦 124010;
2.大连医科大学附属口腔医院口腔科,辽宁大连 116085;
3.盘锦市大洼区中医院口腔科,辽宁盘锦 124200

收稿日期:2022-01-12 修回日期:2022-04-14 出版日期:2022-09-20 发布日期:2022-09-20
通讯作者: 许刚,E-mail: xvgang202105@163.com
作者简介:许刚（1983-）,男,本科,副主任医师

Expression and clinical significance of aquaporin 3 in oral mucosal epithelial dysplasia and oral squamous cell carcinoma

XU Gang¹, SONG Qi-yi², JIANG Wei-dong¹, LIU Jing-xiu³

1. Department of Stomatology, Panjin Liaoyou Baoshihua Hospital. Panjin 124010;
2. Department of Stomatology, Dalian Stomatology Hospital Affiliated of Dalian Medical University. Dalian 116085;
3. Department of Stomatology, Dawa District Hospital of Traditional Chinese Medicine. Panjin 124200, Liaoning Province, China

Received:2022-01-12 Revised:2022-04-14 Online:2022-09-20 Published:2022-09-20

摘要/Abstract

摘要： 目的: 探讨水通道蛋白3（AQP3）在口腔鳞癌中的表达及临床意义。方法: 收集2014年3月—2017年4月盘锦辽油宝石花医院保存的202例口腔黏膜组织石蜡标本。其中健康者41例（对照组）、口腔黏膜上皮异常增生45例（A组）、口腔鳞癌116例（B组）。利用免疫组织化学方法检测3组口腔黏膜组织中AQP3的表达情况,分析口腔鳞癌口腔黏膜组织中AQP3表达与临床病理因素及预后的关系。采用SPSS 18.0软件包对数据进行统计学分析。结果: B组口腔黏膜组织中AQP3阳性表达率显著高于A组和对照组（P<0.05）,A组口腔黏膜组织中AQP3阳性表达率显著高于对照组（P<0.05）。肿瘤侵袭深度>5 mm、临床T4分期、颈淋巴结转移、低分化患者,口腔黏膜组织中AQP3阳性表达率分别高于肿瘤侵袭深度≤5 mm、临床T2分期、无颈淋巴结转移、中高分化患者（P<0.05）。AQP3阳性表达的口腔鳞癌患者,术后3年生存率（65.85%）显著低于阴性表达患者（90.00%,P<0.05）。Cox回归分析显示,肿瘤临床分期、分化程度、颈淋巴结转移情况、AQP3阳性表达率是影响总生存率的独立预后因素（P<0.05）。结论: 口腔鳞癌患者口腔黏膜组织中AQP3阳性表达率显著高于口腔黏膜上皮异常增生者,AQP3表达与口腔鳞癌患者临床分期、颈淋巴结转移、分化程度、肿瘤侵袭深度及总生存率相关。

关键词: 水通道蛋白3, 口腔黏膜上皮异常增生, 口腔鳞癌, 病理, 预后

Abstract: PURPOSE: To explore the expression and clinical significance of aquaporin 3 (AQP3) in oral squamous cell carcinoma(OSCC). METHODS: Two hundred and two oral mucosal tissue paraffin specimens were collected in Panjin Liaoyou Baoshihua Hospital from March 2014 to April 2017. Among them, forty-one healthy volunteers (control group) and 45 oral mucosal epithelial abnormalities (group A) , one hundred and sixteen cases of OSCC(group B) were included. The expression of AQP3 in oral mucosa tissue of the 3 groups was detected by immunohistochemical staining. The relationship between AQP3 expression and clinicopathological factors and prognosis of OSCC were analyzed. SPSS 18.0 software package was used for data analysis. RESULTS: The positive expression rate of AQP3 in group B was significantly higher than that of group A and control group (P<0.05), and AQP3 positive expression rate in oral mucosa tissues of group A was significantly higher than that of the control group(P<0.05). AQP3 positive expression rate in OSCC patients with tumor invasion depth> 5 mm, clinical T4 staging, cervical lymph node metastasis, and poorly differentiated patients were respectively higher than that in patients with tumor invasion depth≤5 mm, clinical T2 staging, no cervical lymph node metastasis, moderately well differentiated patients (P<0.05). The 3-year survival rate of OSCC patients with positive AQP3 expression(65.85%) was significantly lower than patients with negative AQP3 expression(90.00%, P<0.05). Cox regression analysis showed that tumor clinical stage, degree of differentiation, cervical lymph node metastasis, and AQP3 positive expression rate are independent prognostic factors that affect overall survival (P<0.05). CONCLUSION: The positive expression rate of AQP3 in oral mucosal tissues of OSCC patients is higher than that in patients with oral mucosal epithelial dysplasia. The expression of AQP3 is related to clinical stage, cervical lymph node metastasis, degree of differentiation, depth of tumor invasion and overall survival rate in OSCC patients.

Key words: Aquaporin 3, Oral mucosal epithelial hyperplasia, Oral squamous cell carcinoma, Pathology, Prognosis

中图分类号:

R739.8

许刚, 宋其义, 蒋卫东, 刘景秀. 水通道蛋白3在口腔鳞癌中的表达及临床意义[J]. 中国口腔颌面外科杂志, 2022, 20(5): 478-482.

XU Gang, SONG Qi-yi, JIANG Wei-dong, LIU Jing-xiu. Expression and clinical significance of aquaporin 3 in oral mucosal epithelial dysplasia and oral squamous cell carcinoma[J]. China J Oral Maxillofac Surg, 2022, 20(5): 478-482.

参考文献

[1] Ilhan B, Lin K, Guneri P, et al.Improving oral cancer outcomes with imaging and artificial intelligence[J]. J Dent Res, 2020, 99(3): 241-248.
[2] Abati S, Bramati C, Bondi S, et al.Oral cancer and precancer: a narrative review on the relevance of early diagnosis[J]. Int J Environ Res Public Health, 2020, 17(24): 9160-9163.
[3] Seta R, Mascitti M, Campagna R, et al.Overexpression of nicotinamide N-methyltransferase in HSC-2 OSCC cell line: effect on apoptosis and cell proliferation[J]. Clin Oral Investig, 2019, 23(2): 829-838.
[4] Xia H, Ma YF, Yu CH, et al.Aquaporin 3 knockdown suppresses tumour growth and angiogenesis in experimental non-small cell lung cancer[J]. Exp Physiol, 2014, 99(7): 974-984.
[5] Satooka H, Hara-Chikuma M.Aquaporin-3 controls breast cancer cell migration by regulating hydrogen peroxide transport and its downstream cell signaling[J]. Mol Cell Biol, 2016, 36(7): 1206-1218.
[6] Lekshmy MS, Sivakumar TT, Joseph AP, et al.Expression of transmembrane protein aquaporin-3 in oral epithelial dysplasia and oral squamous cell carcinoma[J]. Oral Surg Oral Med Oral Pathol Oral Radiol, 2021, 131(2): 202-208.
[7] 中华口腔医学会口腔病理学专业委员会. 口腔癌及口咽癌病理诊断规范[J]. 中国口腔颌面外科杂志, 2020, 18(4): 289-296.
Professional Committee of Oral Pathology, Chinese Stomatological Association. Guideline on pathological diagnosis of oral and oropharyngeal squamous cell carcinoma[J].China Journal of Oral and Maxillofacial Surgery,2020, 18(4): 289-296.
[8] Chamoli A, Gosavi AS, Shirwadkar UP, et al.Overview of oral cavity squamous cell carcinoma: risk factors, mechanisms, and diagnostics[J]. Oral Oncol, 2021, 121: 105451.
[9] Johnson DE, Burtness B, Leemans CR, et al.Head and neck squamous cell carcinoma[J]. Nat Rev Dis Primers, 2020, 6(1): 92-104.
[10] Moosavi MS, Elham Y.Aquaporins 1, 3 and 5 in different tumors, their expression, prognosis value and role as new therapeutic targets[J]. Pathol Oncol Res, 2020, 26(2): 615-625.
[11] Liu C, Liu L, Zhang Y, et al.Molecular mechanism of aquapontin (AQP3) in regulating differentiation and apoptosis of lung cancer stem cells through Wnt/GSK-3β/β-Catenin pathway[J]. J BUON, 2020, 25(2): 828-834.
[12] Wang S, Wu Y, Yang S, et al.miR-874 directly targets AQP3 to inhibit cell proliferation, mobility and EMT in non-small cell lung cancer[J]. Thorac Cancer, 2020, 11(6): 1550-1558.
[13] Sonntag Y, Gena P, Maggio A, et al.Identification and characterization of potent and selective aquaporin-3 and aquaporin-7 inhibitors[J]. J Biol Chem, 2019, 294(18): 7377-7387.
[14] 张泽桐, 房朗, 甘翼博, 等. 水通道蛋白3在高渗环境诱导髓核细胞凋亡中的作用[J]. 第三军医大学学报, 2020, 42(9):66-73.
Zhang ZT, Fang L, Gan YB, et al.Role of aquaporin-3 in hyperosmolarity-induced apoptosis of rat nucleus pulposus cells in vitro[J]. Journal of Third Military Medical University, 2020, 42(9): 915-922.
[15] Udompatanakorn C, Yada N, Matsuo K.Assessing the expression of aquaporin 3 antigen-recognition sites in oral squamous cell carcinoma[J]. Appl Immunohistochem Mol Morphol, 2020, 28(8): 611-620.
[16] Hong Y, Chen Z, Li N, et al.Prognostic value of serum aquaporin-1, aquaporin-3 and galectin-3 for young patients with colon cancer[J]. Ann Clin Biochem, 2020, 57(6): 404-411.
[17] Xu S, Huang S, Li D, et al.The expression of aquaporin-1 and aquaporin-3 in extrahepatic cholangiocarcinoma and their clinicopathological significance[J]. Am J Med Sci, 2021, 364(2):181-191.
[18] Kusayama M, Wada K, Nagata M, et al.Critical role of aquaporin 3 on growth of human esophageal and oral squamous cell carcinoma[J]. Cancer Sci, 2011, 102(6): 1128-1136.

水通道蛋白3在口腔鳞癌中的表达及临床意义

Expression and clinical significance of aquaporin 3 in oral mucosal epithelial dysplasia and oral squamous cell carcinoma

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	苏姿尚, 崔文丽, 张梦云, 姚志涛. 口腔鳞状细胞癌患者颈部淋巴结特征与预后的关系[J]. 中国口腔颌面外科杂志, 2023, 21(1): 41-48.
[2]	刘恒, 段晓峰. 铁自噬及相关基因NCOA4、FTH1在口腔鳞癌中的研究进展[J]. 中国口腔颌面外科杂志, 2023, 21(1): 87-91.
[3]	杨慧丽, 王宇帆, 王锋, 沈月洪, 杨宏宇. NLRP1在头颈部鳞癌中的表达及预后意义[J]. 中国口腔颌面外科杂志, 2022, 20(5): 429-436.
[4]	季萍萍, 李莉, 吴奕帆, 侯黎莉. 经皮穴位电刺激防治口腔鳞癌患者诱导化疗后骨髓抑制的效果观察[J]. 中国口腔颌面外科杂志, 2022, 20(5): 455-460.
[5]	赵小妹, 夏荣辉, 钟来平, 朱东旺, 张志愿. Nrf2对口腔鳞癌患者TPF诱导化疗效果的影响[J]. 中国口腔颌面外科杂志, 2022, 20(4): 366-370.
[6]	杨宋, 王宁, 赵树超, 李恒勇, 葛胜优, 周凯, 宋凯, 尚伟, 冯元勇. Chemerin在合并2型糖尿病口腔鳞癌患者肿瘤中的表达及临床意义[J]. 中国口腔颌面外科杂志, 2022, 20(4): 390-395.
[7]	张益益, 杨玥怡, 琚梧桐, 钟来平. 局部晚期口腔鳞癌新辅助免疫靶向治疗2例及文献复习[J]. 中国口腔颌面外科杂志, 2022, 20(4): 413-416.
[8]	魏东亮, 李智, 鞠侯雨, 吴云腾, 郭伟, 任国欣. EGFR靶向药物对口腔癌疼痛缓解作用的机制探讨[J]. 中国口腔颌面外科杂志, 2022, 20(3): 219-224.
[9]	杨金刚, 夏荣辉, 朱云, 吴思成, 董亚兵, 杨功鑫, 史俊隆, 崔迎慧, 朱凌, 周晌辉. 98例原发性颌骨内鳞状细胞癌预后影响因素的回顾性分析[J]. 中国口腔颌面外科杂志, 2022, 20(3): 239-243.
[10]	徐卫靖, 高振杰, 付坤, 高宁, 程龙, 何巍. 33例头颈部胚胎型横纹肌肉瘤临床与病理特点分析[J]. 中国口腔颌面外科杂志, 2022, 20(3): 292-297.
[11]	孙大为, 张云, 朱勇. shRNA干扰Rce1基因表达对口腔鳞癌细胞放射敏感性的影响[J]. 中国口腔颌面外科杂志, 2022, 20(2): 123-128.
[12]	努尔曼古丽·牙森, 多力昆·吾甫尔, 白尔娜·吾守尔, 闫广鹏. 血清IL-6、IL-17、TNF-α和IFN-γ水平与60例口腔鳞癌患者临床特征关系探讨[J]. 中国口腔颌面外科杂志, 2022, 20(2): 141-145.
[13]	陈一铭, Ahmed, 季彤, 任振虎. 101例头颈部横纹肌肉瘤临床、病理及预后分析[J]. 中国口腔颌面外科杂志, 2022, 20(2): 146-150.
[14]	瞿楚翔, 孙树洋, 张志愿. 从遗传异质性认识口腔鳞癌的侵袭过程[J]. 中国口腔颌面外科杂志, 2022, 20(2): 193-197.
[15]	王育新, 王志勇, 王永功, 王慧明, 王丽珍, 田皞, 叶金海, 付坤, 阮敏, 孙长伏, 孙国文, 刘法昱, 许碧云, 后军, 刘冰, 刘亮, 陈永锋, 陈占伟, 肖灿, 李思毅, 李志勇, 李勇, 李楠, 张陈平, 张东升, 张胜, 张凯, 杨溪, 何巍, 苏彤, 季彤, 林李嵩, 罗瑞华, 赵志立, 胡勤刚, 唐瞻贵, 徐蔚嘉, 黄志权, 黄晓峰, 梁玉洁, 韩正学, 韩伟, 蒋灿华, 喻建军, 蔡志刚, 廖贵清, 廖圣恺, 蔡惠明, 魏建华. 吲哚菁绿荧光成像技术在口腔鳞癌治疗中的应用：中国专家共识[J]. 中国口腔颌面外科杂志, 2022, 20(1): 1-6.