中国口腔颌面外科杂志

中国口腔颌面外科杂志 ›› 2022, Vol. 20 ›› Issue (3): 251-256.doi: 10.19438/j.cjoms.2022.03.008

• 论著 • 上一篇    下一篇

口腔种植体周围炎患者牙龈中组织蛋白酶B及Beclin-1的表达及临床意义

薛秋波1, 薛陆峰1, 马成1, 丁皓月2   

  1. 1.中国人民解放军联勤保障部队第九四医院 口腔科,江苏 无锡 214000;
    2.无锡市妇幼保健院 口腔科,江苏 无锡 214000
  • 收稿日期:2021-10-02 修回日期:2022-01-04 出版日期:2022-05-20 发布日期:2022-05-20
  • 通讯作者: 薛陆峰,E-mail: x2248929xiep@163.com
  • 作者简介:薛秋波（1990-）,男,本科,E-mail: xqb18036009167@163.com

Expression, clinical significance of cathepsin B, Beclin-1in the gingival tissues of patients with peri-implantitis

XUE Qiu-bo1, XUE Lu-feng1, MA Cheng1, DING Hao-yue2   

  1. 1. Department of Stomatology, No. 904 Hospital of Joint Service Support Force of the Chinese People's Liberation Army. Wuxi 214000;
    2. Department of Stomatology, Wuxi Maternal and Child Health Hospital. Wuxi 214000, Jiangsu Province, China
  • Received:2021-10-02 Revised:2022-01-04 Online:2022-05-20 Published:2022-05-20

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摘要： 目的: 探讨口腔种植体周围炎患者牙龈中组织蛋白酶B（Cat B）及Beclin-1的表达及临床意义。方法: 选择124例口腔种植体周围炎患者病变牙龈组织作为实验组,124例健康牙龈组织（阻生牙拔除）为对照组,分别采用实时荧光定量PCR和蛋白免疫印迹法（Western blot）检测Cat B和Beclin-1 mRNA及蛋白表达量。统计术后1年口腔种植体周围炎患者种植体松动、脱落情况。Logistic多因素回归分析影响口腔种植体周围炎患者种植体松动/脱落的有关因素。制作受试者工作特征曲线（ROC）,分析牙龈组织中Cat B、Beclin-1 mRNA表达量对口腔种植体周围炎患者种植体松动/脱落发生的预测价值。采用SPSS 21.0软件包对数据进行统计学分析。结果: 实验组牙龈组织中Cat B、Beclin-1 mRNA及蛋白表达量均显著高于对照组（P<0.05）。口腔种植体周围炎患者术后1年种植体松动/脱落发生率为16.94%。松动/脱落组合并糖尿病占比、吸烟史占比、种植体负载时间、降钙素原（PCT）、牙龈组织中Cat B及Beclin-1 mRNA表达量显著高于未松动/脱落组（P<0.05）。Logistic多因素分析结果显示,PCT、Cat B 及Beclin-1 mRNA均为影响口腔种植体周围炎患者种植体松动/脱落的危险因素（OR=2.751、3.414、3.196,P<0.05）。ROC分析显示,牙龈组织中Cat B、Beclin-1 mRNA表达量对口腔种植体周围炎患者种植体松动/脱落发生进行预测的最佳截断点分别为1.42、2.51,灵敏度分别为61.90%、76.19%,特异度分别为76.70%、60.19%,AUC分别为0.728、0.771。结论: 与牙龈健康者相比,口腔种植体周围炎患者牙龈组织中Cat B、Beclin-1 mRNA均呈高表达,且牙龈组织中Cat B、Beclin-1 mRNA表达量对口腔种植体周围炎患者种植体松动/脱落发生的预测价值较高,可作为临床评估种植体松动/脱落发生的重要参考指标。

关键词: 种植体周围炎, 组织蛋白酶B, Beclin-1, 种植体松动, 种植体脱落

Abstract: PURPOSE: To explore the changes and clinical significance of cathepsin B (Cat B) and Beclin-1 expression in the gingival tissues of patients with peri-implantitis. METHODS: One hundred and twenty-four cases with peri-implantitis were collected as the experimental group, and 124 cases of healthy gingival tissues (due to impacted tooth extraction) as the control group. The expression of Cat B and Beclin-1 mRNA and protein in the gingival tissues of the two groups were detected by RT-PCR and Western blot, respectively. One year after operation, the patients with peri-implantitis were classified into loosing/dropping group and non-loosing/dropping group. The clinical data of patients in the two groups were analyzed. Logistic multivariate regression analysis was conducted to evaluate related factors affecting implant loosing/dropping in patients with peri-implantitis. A receiver operating characteristic curve (ROC) was made to analyze the predictive value of Cat B and Beclin-1 mRNA expression in gingival tissue for the occurrence of implant loosing/dropping in patients with peri-implantitis. SPSS21.0 software package was used for data analysis. RESULTS: Cat B and Beclin-1 mRNA and protein expression in the gingival tissues of the experimental group was significantly higher than those of the control group(P<0.05). The incidence of implant loosing/dropping in patients with peri-implantitis was 16.94% one year after operation. The prevalence of diabetes, smoking history, implant loading time, procalcitonin(PCT), and the expression of Cat B and Beclin-1 mRNA in gingival tissue in the loosing/dropping group were significantly higher than those in the non-loosing/dropping group (P<0.05). Logistic multivariate analysis showed that PCT, Cat B and Beclin-1 mRNA were risk factors affecting the occurrence of implant loosing/dropping in patients with peri-implantitis (OR=2.751, 3.414, 3.196, P<0.05). ROC analysis showed that the best cut-off points for the expression of Cat B and Beclin-1 mRNA in gingival tissue to predict the occurrence of implant loosing/dropping in patients with peri-implantitis were 1.42 and 2.51, respectively, and the sensitivities were 61.90% and 76.19%, respectively; specificity was 76.70%, 60.19%, and AUC was 0.728 and 0.771, respectively. CONCLUSIONS: Compared with healthy gingiva, Cat B and Beclin-1 mRNA in the gingival tissues of patients with peri-implantitis are both highly expressed, and the expression of Cat B and Beclin-1 mRNA in the gingival tissues has an effect on peri-implant inflammation. The predictive value of the occurrence of implant loosening/dropping in patients is high, and it can be used as an important reference index for clinical evaluation of the occurrence of implant loosing/dropping.

Key words: Peri-implantitis, Cathepsin B, Beclin-1, Implant loosing, Implant dropping

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